Use of PLL-g-PEG in micro-fluidic devices for localizing selective and specific protein binding

Use of PLL-g-PEG in micro-fluidic devices for localizing selective and specific protein binding

By utilizing flow-controlled PLL-g-PEG and PLL-g-PEGbiotin modification of predefined regions of a poly(dimethylsiloxane) (PDMS) micro-fluidic device, with an intentionally chosen large (approximately 1 cm2) internal surface area, we report rapid (10 min), highly localized (6 x 10(-6) cm2), and spec...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1991. - 22(2006), 24 vom: 21. Nov., Seite 10103-8
1. Verfasser: Marie, Rodolphe (VerfasserIn)
Weitere Verfasser: Beech, Jason P, Vörös, Janos, Tegenfeldt, Jonas O, Höök, Fredrik
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Dimethylpolysiloxanes Polymers Silicones Polylysine 25104-18-1 Polyethylene Glycols 3WJQ0SDW1A baysilon mehr... 63148-62-9 Propylene Glycol 6DC9Q167V3 Biotin 6SO6U10H04 Streptavidin 9013-20-1
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245 1 0 |a Use of PLL-g-PEG in micro-fluidic devices for localizing selective and specific protein binding 
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500 |a Date Revised 01.12.2018 
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520 |a By utilizing flow-controlled PLL-g-PEG and PLL-g-PEGbiotin modification of predefined regions of a poly(dimethylsiloxane) (PDMS) micro-fluidic device, with an intentionally chosen large (approximately 1 cm2) internal surface area, we report rapid (10 min), highly localized (6 x 10(-6) cm2), and specific surface-based protein capture from a sample volume (100 microL) containing a low amount of protein (160 attomol in pure buffer and 400 attomol in serum). The design criteria for this surface modification were achieved using QCM-D (quartz crystal microbalance with energy dissipation monitoring) of serum protein adsorption onto PLL-g-PEG-modified oxidized PDMS. Equally good, or almost as good, results were obtained for oxidized SU-8, Topas, and poly(methyl metacrylate) (PMMA), demonstrating the generic potential of PLL-g-PEG for surface modification in various micro-fluidic applications 
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700 1 |a Beech, Jason P  |e verfasserin  |4 aut 
700 1 |a Vörös, Janos  |e verfasserin  |4 aut 
700 1 |a Tegenfeldt, Jonas O  |e verfasserin  |4 aut 
700 1 |a Höök, Fredrik  |e verfasserin  |4 aut 
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