Overexpression of an Arabidopsis magnesium transport gene, AtMGT1, in Nicotiana benthamiana confers Al tolerance

Aluminium (Al) toxicity is the most important limiting factor for crop production in acid soil environments worldwide. In some plant species, application of magnesium (Mg(2+)) can alleviate Al toxicity. However, it remains unknown whether overexpression of magnesium transport proteins can improve Al...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 57(2006), 15 vom: 05., Seite 4235-43
1. Verfasser: Deng, Wei (VerfasserIn)
Weitere Verfasser: Luo, Keming, Li, Demou, Zheng, Xuelian, Wei, Xiaoyang, Smith, William, Thammina, Chandra, Lu, Litang, Li, Yi, Pei, Yan
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Arabidopsis Proteins Cation Transport Proteins Glucans MGT1 protein, Arabidopsis Manganese 42Z2K6ZL8P callose mehr... 9064-51-1 Aluminum CPD4NFA903 Iron E1UOL152H7 Magnesium I38ZP9992A
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245 1 0 |a Overexpression of an Arabidopsis magnesium transport gene, AtMGT1, in Nicotiana benthamiana confers Al tolerance 
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520 |a Aluminium (Al) toxicity is the most important limiting factor for crop production in acid soil environments worldwide. In some plant species, application of magnesium (Mg(2+)) can alleviate Al toxicity. However, it remains unknown whether overexpression of magnesium transport proteins can improve Al tolerance. Here, the role of AtMGT1, a member of the Arabidopsis magnesium transport family involved in Mg(2+) transport, played in Al tolerance in higher plants was investigated. Expression of 35S::AtMGT1 led to various phenotypic alterations in Nicotiana benthamiana plants. Transgenic plants harbouring 35S::AtMGT1 exhibited tolerance to Mg(2+) deficiency. Element assay showed that the contents of Mg, Mn, and Fe in 35S::AtMGT1 plants increased compared with wild-type plants. Root growth experiment revealed that 100 microM AlCl(3) caused a reduction in root elongation by 47% in transgenic lines, whereas root growth in wild-type plants was inhibited completely. Upon Al treatment, representative transgenic lines also showed a much lower callose deposition, an indicator of increased Al tolerance, than wild-type plants. Taken together, the results have demonstrated that overexpression of ATMGT1 encoding a magnesium transport protein can improve tolerance to Al in higher plants 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
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700 1 |a Luo, Keming  |e verfasserin  |4 aut 
700 1 |a Li, Demou  |e verfasserin  |4 aut 
700 1 |a Zheng, Xuelian  |e verfasserin  |4 aut 
700 1 |a Wei, Xiaoyang  |e verfasserin  |4 aut 
700 1 |a Smith, William  |e verfasserin  |4 aut 
700 1 |a Thammina, Chandra  |e verfasserin  |4 aut 
700 1 |a Lu, Litang  |e verfasserin  |4 aut 
700 1 |a Li, Yi  |e verfasserin  |4 aut 
700 1 |a Pei, Yan  |e verfasserin  |4 aut 
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