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231223s2007 xx ||||| 00| ||eng c |
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|a pubmed24n0554.xml
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|a (DE-627)NLM166301531
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|a (NLM)17074416
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Shishova, Maria
|e verfasserin
|4 aut
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|a A shift in sensitivity to auxin within development of maize seedlings
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|c 2007
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 04.01.2008
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The auxin-induced changes in cytosolic concentrations of Ca(2+) and H(+) ions were investigated in protoplasts from maize coleoptile cells at 3rd, 4th and 5th day of development of etiolated seedlings. The shifts in [Ca(2+)](cyt) and [H(+)](cyt) were detected by use of fluorescence microscopy in single protoplasts loaded with the tetra[acetoxymethyl]esters of the fluorescent calcium binding Fura 2, or pH-sensitive carboxyfluorescein, BCECF, respectively. Both the auxin-induced shifts in the ion concentrations were specific to the physiologically active synthetic auxin, naphthalene-1-acetic acid (1-NAA), and not to the non-active naphthalene-2-acetic acid (2-NAA). Regardless of the age of the seedlings, the rise in [Ca(2+)](cyt) was prior to the acidification in all investigated cases. The maximal acidification coincided with the highest amplitude of [Ca(2+)](cyt) change, but not directly depended on the concentration of 1-NAA. Within aging of the seedlings the amplitude of auxin-induced [Ca(2+)](cyt) elevation decreased. The shift in auxin-induced acidification was almost equal at 3rd and 4th day, but largely dropped at 5th day of development. The acidification was related to changes in the plasma membrane H(+)-ATPase activity, detected as phosphate release. The decrement in amplitude of both the tested auxin-triggered reactions well coincided with the end of the physiological function of the coleoptile. Hence the primary auxin-induced increase in [Ca(2+)](cyt), which is supposed to be an important element of hormone signal perception and transduction, can be used as a test for elucidation of plant cell sensitivity to auxin
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Indoleacetic Acids
|2 NLM
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|a Naphthaleneacetic Acids
|2 NLM
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|a 1-naphthaleneacetic acid
|2 NLM
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|a 33T7G7757C
|2 NLM
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|a Hydrogen
|2 NLM
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|a 7YNJ3PO35Z
|2 NLM
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|a Calcium
|2 NLM
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|a SY7Q814VUP
|2 NLM
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| 700 |
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|a Yemelyanov, Vladislav
|e verfasserin
|4 aut
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|a Rudashevskaya, Elena
|e verfasserin
|4 aut
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| 700 |
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|a Lindberg, Sylvia
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 164(2007), 10 vom: 15. Okt., Seite 1323-30
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:164
|g year:2007
|g number:10
|g day:15
|g month:10
|g pages:1323-30
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|d 164
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|h 1323-30
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