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NLM166269956 |
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DE-627 |
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20250207175700.0 |
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231223s2006 xx ||||| 00| ||eng c |
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|a pubmed25n0554.xml
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|a (DE-627)NLM166269956
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|a (NLM)17071097
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Feng, B
|e verfasserin
|4 aut
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| 245 |
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|a Isolation of a novel Ser/Thr protein kinase gene from oligochitosan-induced tobacco and its role in resistance against tobacco mosaic virus
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|c 2006
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 01.02.2007
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Oligochitosan induces defense responses to pathogenic microbes in a wide variety of plants by acting as an elicitor. In the present study, mRNA differential display was used to investigate oligochitosan-induced transcriptional activation of defense-related genes. Accordingly, a novel Ser/Thr protein kinase gene was isolated and designated as oligochitosan-induced protein kinase (oipk). Molecular cloning showed that oipk contains six introns interrupted by seven exons. The open reading frame (ORF) of the gene is 1848 bp, which encodes a putative protein of 615 amino acids with the predicted molecular mass of 70.96 kDa and a pI of 6.32. A plant oipk antisense expression vector was constructed and transformed into tobacco by Agrobacterium tumefaciens. Decreased phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) activity and decreased resistance to tobacco mosaic virus (TMV) were observed in transgenic tobacco. RT-PCR analysis revealed that oipk was expressed at high levels after oligochitosan induction in wild-type tobacco, but not in transgenic tobacco. These results indicated that oipk is involved in the signal pathway of oligochitosan-induced resistance in tobacco
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Oligosaccharides
|2 NLM
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|a oligochitosan
|2 NLM
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| 650 |
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|a Chitin
|2 NLM
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7 |
|a 1398-61-4
|2 NLM
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| 650 |
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|a Chitosan
|2 NLM
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| 650 |
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7 |
|a 9012-76-4
|2 NLM
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| 650 |
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|a Protein Serine-Threonine Kinases
|2 NLM
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| 650 |
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|a EC 2.7.11.1
|2 NLM
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| 700 |
1 |
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|a Chen, Y
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhao, C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhao, X
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bai, X
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Du, Y
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 44(2006), 10 vom: 15. Okt., Seite 596-603
|w (DE-627)NLM098178261
|x 0981-9428
|7 nnns
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| 773 |
1 |
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|g volume:44
|g year:2006
|g number:10
|g day:15
|g month:10
|g pages:596-603
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|d 44
|j 2006
|e 10
|b 15
|c 10
|h 596-603
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