Antioxidative responses to mercury in the cell cultures of Sesbania drummondii

The effect of mercury (Hg) on the growth and the response of antioxidative systems have been investigated in Sesbania cell cultures to determine the tolerance limits and the mechanisms of metal (Hg) tolerance in plant cells. Cell cultures of Sesbania were developed in different concentrations (0-50...

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Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 44(2006), 10 vom: 05. Okt., Seite 590-5
1. Verfasser: Israr, Mohd (VerfasserIn)
Weitere Verfasser: Sahi, Shivendra V
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Antioxidants Sulfhydryl Compounds Superoxide Dismutase EC 1.15.1.1 Mercury FXS1BY2PGL Glutathione GAN16C9B8O
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520 |a The effect of mercury (Hg) on the growth and the response of antioxidative systems have been investigated in Sesbania cell cultures to determine the tolerance limits and the mechanisms of metal (Hg) tolerance in plant cells. Cell cultures of Sesbania were developed in different concentrations (0-50 microM) of mercury. Cultures tolerated Hg up to a concentration of 40 microM and showed an increase in the fresh weight growth by 620% in 3 weeks. The levels of antioxidants: glutathione (GSH) and non-protein thiols (NPSH) and the activities of antioxidative enzymes: superoxide dismutase (SOD, EC 1.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2) were influenced by Hg treatments. The contents of GSH, NPSH and GSH/GSSG ratio increased up to a concentration of 40 muM Hg and then severely declined at 50 microM Hg. The activities of antioxidative enzymes, SOD, APX and GR followed the same trends as antioxidants, first increased up to a concentration of 40 muM Hg and then declined in the presence of 50 microM Hg 
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