Microplate assay for quantitative determination of cathepsin activities in viable cells using derivatives of 4-methoxy-beta-naphthylamide

A method is described allowing the selective determination of four cathepsins (B, H, K, and L) in live cells. Adherently growing cells are incubated with partially selective substrates for each cathepsin (peptidic derivatives of 4-methoxy-beta-naphthylamine) in microtiter plates together with nitros...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 41(2006), 4 vom: 27. Okt., Seite 469-73
1. Verfasser: Rüttger, Anke (VerfasserIn)
Weitere Verfasser: Mollenhauer, Jürgen, Löser, Reik, Gütschow, Michael, Wiederanders, Bernd
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Comparative Study Journal Article Research Support, Non-U.S. Gov't Benzaldehydes Enzyme Inhibitors 5-nitrosalicylaldehyde 97-51-8 2-Naphthylamine CKR7XL41N4 Cathepsins mehr... EC 3.4.- Aminopeptidases EC 3.4.11.- Cysteine Endopeptidases EC 3.4.22.- Cathepsin B EC 3.4.22.1 CTSL protein, human EC 3.4.22.15 Cathepsin L Ctsl protein, mouse CTSH protein, human EC 3.4.22.16 Cathepsin H Ctsh protein, mouse CTSK protein, human EC 3.4.22.38 Cathepsin K Ctsk protein, mouse Leucine GMW67QNF9C ubenimex I0J33N5627