Improved DNA methylation analysis via enrichment of demethylated cells expressing an X-inactivated transgene
The demethylating drug 5-aza-2'-deoxycytidine (5-aza-2dC) is frequently used to investigate the effect of global DNA demethylation on gene expression in cultured mammalian cells. Here, we describe a method that uses the reactivation of an X-inactivated green fluorescent protein (GFP) transgene...
| Publié dans: | BioTechniques. - 1991. - 41(2006), 4 vom: 27. Okt., Seite 461-6 |
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| Format: | Article |
| Langue: | English |
| Publié: |
2006
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Technical Report Green Fluorescent Proteins 147336-22-9 Azacitidine M801H13NRU |