Improved DNA methylation analysis via enrichment of demethylated cells expressing an X-inactivated transgene
The demethylating drug 5-aza-2'-deoxycytidine (5-aza-2dC) is frequently used to investigate the effect of global DNA demethylation on gene expression in cultured mammalian cells. Here, we describe a method that uses the reactivation of an X-inactivated green fluorescent protein (GFP) transgene...
| Veröffentlicht in: | BioTechniques. - 1991. - 41(2006), 4 vom: 27. Okt., Seite 461-6 |
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| Format: | Aufsatz |
| Sprache: | English |
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2006
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Technical Report Green Fluorescent Proteins 147336-22-9 Azacitidine M801H13NRU |
| Zusammenfassung: | The demethylating drug 5-aza-2'-deoxycytidine (5-aza-2dC) is frequently used to investigate the effect of global DNA demethylation on gene expression in cultured mammalian cells. Here, we describe a method that uses the reactivation of an X-inactivated green fluorescent protein (GFP) transgene as a marker to enrich for cells that have undergone drug-induced demethylation. By combining it with microarray gene expression profiling, we demonstrate the method's utility in identifying genes activated by global DNA demethylation |
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| Beschreibung: | Date Completed 22.11.2006 Date Revised 07.06.2018 published: Print Citation Status MEDLINE |
| ISSN: | 1940-9818 |