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231223s2006 xx ||||| 00| ||eng c |
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|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Simpson, Joyce M
|e verfasserin
|4 aut
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| 245 |
1 |
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|a 16S ribosomal RNA tools identify an unexpected predominance of Paenibacillus-like bacteria in an industrial activated sludge system suffering from poor biosolids separation
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|c 2006
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
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|2 rdamedia
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|b nc
|2 rdacarrier
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|a Date Completed 07.02.2007
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|a Date Revised 22.09.2019
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|a published: Print
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|a Citation Status MEDLINE
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|a Molecular biology tools targeting 16S ribosomal RNA (16S rRNA) were used to identify a predominant bacterial population in a full-scale dairy wastewater activated sludge system suffering from poor biosolids separation. Gram and acridine orange staining indicated that viable, Gram-positive microorganisms were present in samples removed from the influent waste stream and represented approximately 50% of total cell counts in samples removed from the mixed liquor. Subsequently, the "full-cycle 16S rRNA approach" showed that phylogenetic relatives of Paenibacillus spp., a low guanine-plus-cytosine percent DNA-content, Gram-positive microorganism, represented up to 30% of total 4,6-diamidino-2-phenylindole (DAPI)-stained cell counts in samples of mixed liquor. Although fluorescent in situ hybridizations with 16S rRNA-targeted oligonucleotide hybridization probes identified Paenibacillus-like spp. in samples removed from the influent waste stream, their abundance was less than 10% of total stained cell counts. Results of this study suggest that Paenibacillus-like spp. were present in low abundance in the influent waste stream, increased in relative abundance within the treatment system, and should be examined further as a candidate bacterial population responsible for poor biosolids separation. This study demonstrates that the full-cycle 16S rRNA approach can be used to identify candidate bacterial populations that may be responsible for operational upsets in full-scale activated sludge systems without prior information from cultivation or microscopic analyses
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|a Journal Article
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|a Industrial Waste
|2 NLM
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| 650 |
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|a RNA, Bacterial
|2 NLM
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| 650 |
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|a RNA, Ribosomal, 16S
|2 NLM
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| 700 |
1 |
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|a Stroot, Peter G
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gelman, Steve
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Beydilli, Inan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Dudley, Sandra
|e verfasserin
|4 aut
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| 700 |
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|a Oerther, Daniel B
|e verfasserin
|4 aut
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| 773 |
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8 |
|i Enthalten in
|t Water environment research : a research publication of the Water Environment Federation
|d 1998
|g 78(2006), 8 vom: 30. Aug., Seite 864-71
|w (DE-627)NLM098214292
|x 1554-7531
|7 nnns
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| 773 |
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|g volume:78
|g year:2006
|g number:8
|g day:30
|g month:08
|g pages:864-71
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