Respiratory carbon metabolism in the high mountain plant species Ranunculus glacialis

Very little is known about the primary carbon metabolism of the high mountain plant Ranunculus glacialis. It is a species with C3 photosynthesis, but with exceptionally high malate content in its leaves, the biological significance of which remains unclear. 13C/12C-isotope ratio mass spectrometry (I...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 57(2006), 14 vom: 08., Seite 3837-45
1. Verfasser: Nogués, Salvador (VerfasserIn)
Weitere Verfasser: Tcherkez, Guillaume, Streb, Peter, Pardo, Antoni, Baptist, Florence, Bligny, Richard, Ghashghaie, Jaleh, Cornic, Gabriel
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Malates Carbon Dioxide 142M471B3J Carbon 7440-44-0 malic acid 817L1N4CKP
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520 |a Very little is known about the primary carbon metabolism of the high mountain plant Ranunculus glacialis. It is a species with C3 photosynthesis, but with exceptionally high malate content in its leaves, the biological significance of which remains unclear. 13C/12C-isotope ratio mass spectrometry (IRMS) and 13C-nuclear magnetic resonance (NMR) labelling were used to study the carbon metabolism of R. glacialis, paying special attention to respiration. Although leaf dark respiration was high, the temperature response had a Q10 of 2, and the respiratory quotient (CO2 produced divided by O2 consumed) was nearly 1, indicating that the respiratory pool is comprised of carbohydrates. Malate, which may be a large carbon substrate, was not respired. However, when CO2 fixed by photosynthesis was labelled, little labelling of the CO2 subsequently respired in the dark was detected, indicating that: (i) most of the carbon recently assimilated during photosynthesis is not respired in the dark; and (ii) the carbon used for respiration originates from (unlabelled) reserves. This is the first demonstration of such a low metabolic coupling of assimilated and respired carbon in leaves. The biological significance of the uncoupling between assimilation and respiration is discussed 
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700 1 |a Tcherkez, Guillaume  |e verfasserin  |4 aut 
700 1 |a Streb, Peter  |e verfasserin  |4 aut 
700 1 |a Pardo, Antoni  |e verfasserin  |4 aut 
700 1 |a Baptist, Florence  |e verfasserin  |4 aut 
700 1 |a Bligny, Richard  |e verfasserin  |4 aut 
700 1 |a Ghashghaie, Jaleh  |e verfasserin  |4 aut 
700 1 |a Cornic, Gabriel  |e verfasserin  |4 aut 
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