Heat-inducible autolytic vector for high-throughput screening
In directed evolution, a high-throughput screening system is often a prerequisite for sampling the enzyme variants. When the target enzyme is expressed intracellularly, for example when Escherichia coli is used as the host, chemical or enzymatic disruption of cell membrane is often required in many...
Veröffentlicht in: | BioTechniques. - 1991. - 41(2006), 3 vom: 15. Sept., Seite 319-23 |
---|---|
1. Verfasser: | |
Weitere Verfasser: | , , , |
Format: | Aufsatz |
Sprache: | English |
Veröffentlicht: |
2006
|
Zugriff auf das übergeordnete Werk: | BioTechniques |
Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Peptidoglycan beta-Galactosidase EC 3.2.1.23 |