Using bicellar mixtures to form supported and suspended lipid bilayers on silicon chips

Bicellar mixtures, planar lipid bilayer assemblies comprising long- and short-chain phosphatidylcholine lipids in suspension, were used to form supported lipid bilayers on flat silicon substrate and on nanotextured silicon substrates containing arrays of parallel troughs (170 nm wide, 380 nm deep, a...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 22(2006), 19 vom: 12. Sept., Seite 8163-8
1. Verfasser: Zeineldin, Reema (VerfasserIn)
Weitere Verfasser: Last, Julie A, Slade, Andrea L, Ista, Linnea K, Bisong, Paul, O'Brien, Michael J, Brueck, S R J, Sasaki, Darryl Y, Lopez, Gabriel P
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. Lipid Bilayers Phosphatidylcholines Silicon Z4152N8IUI
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245 1 0 |a Using bicellar mixtures to form supported and suspended lipid bilayers on silicon chips 
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520 |a Bicellar mixtures, planar lipid bilayer assemblies comprising long- and short-chain phosphatidylcholine lipids in suspension, were used to form supported lipid bilayers on flat silicon substrate and on nanotextured silicon substrates containing arrays of parallel troughs (170 nm wide, 380 nm deep, and 300 nm apart). Confocal fluorescence and atomic force microscopies were used to characterize the resulting lipid bilayer. Formation of a continuous biphasic undulating lipid bilayer membrane, where the crests and troughs corresponded to supported and suspended lipid bilayer regions, is demonstrated. The use of interferometric lithography to fabricate nanotexured substrates provides an advantage over other nanotextured substrates such as nanoporous alumina by offering flexibility in designing different geometries for suspending lipid bilayers 
650 4 |a Journal Article 
650 4 |a Research Support, N.I.H., Extramural 
650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
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650 7 |a Phosphatidylcholines  |2 NLM 
650 7 |a Silicon  |2 NLM 
650 7 |a Z4152N8IUI  |2 NLM 
700 1 |a Last, Julie A  |e verfasserin  |4 aut 
700 1 |a Slade, Andrea L  |e verfasserin  |4 aut 
700 1 |a Ista, Linnea K  |e verfasserin  |4 aut 
700 1 |a Bisong, Paul  |e verfasserin  |4 aut 
700 1 |a O'Brien, Michael J  |e verfasserin  |4 aut 
700 1 |a Brueck, S R J  |e verfasserin  |4 aut 
700 1 |a Sasaki, Darryl Y  |e verfasserin  |4 aut 
700 1 |a Lopez, Gabriel P  |e verfasserin  |4 aut 
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