|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM164904832 |
| 003 |
DE-627 |
| 005 |
20231223103539.0 |
| 007 |
tu |
| 008 |
231223s2006 xx ||||| 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed24n0550.xml
|
| 035 |
|
|
|a (DE-627)NLM164904832
|
| 035 |
|
|
|a (NLM)16922552
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Roy, Raghunath
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Tuning substrate selectivity of a cationic enzyme using cationic polymers
|
| 264 |
|
1 |
|c 2006
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
|
| 338 |
|
|
|a Band
|b nc
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 21.09.2007
|
| 500 |
|
|
|a Date Revised 24.11.2016
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Noncovalent interactions between an artificial molecular scaffold and a protein are interesting due to the possibility of reversible modulation of the activity of the protein. alpha-Chymotrypsin is a positively charged protein that has been shown to interact with negatively charged polymers. Here we show that positively charged polymers are also capable of electrostatically binding to this protein. The resulting experiments show that the ability of a polymer to bind a protein does not depend only on the pI of the protein. We also realized that the variations in charge density in the polymer backbone afford different selectivities of the enzyme toward charged substrates
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
|
| 650 |
|
7 |
|a Cations
|2 NLM
|
| 650 |
|
7 |
|a Polymers
|2 NLM
|
| 650 |
|
7 |
|a Chymotrypsin
|2 NLM
|
| 650 |
|
7 |
|a EC 3.4.21.1
|2 NLM
|
| 650 |
|
7 |
|a alpha-chymotrypsin
|2 NLM
|
| 650 |
|
7 |
|a EC 3.4.21.1
|2 NLM
|
| 650 |
|
7 |
|a Lysine
|2 NLM
|
| 650 |
|
7 |
|a K3Z4F929H6
|2 NLM
|
| 700 |
1 |
|
|a Sandanaraj, Britto S
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Klaikherd, Akamol
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Thayumanavan, S
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 22(2006), 18 vom: 29. Aug., Seite 7695-700
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
|
| 773 |
1 |
8 |
|g volume:22
|g year:2006
|g number:18
|g day:29
|g month:08
|g pages:7695-700
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_721
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 22
|j 2006
|e 18
|b 29
|c 08
|h 7695-700
|