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231223s2006 xx ||||| 00| ||eng c |
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Vahlberg, Cecilia
|e verfasserin
|4 aut
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| 245 |
1 |
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|a alpha(2A)-adrenergic receptor derived peptide adsorbates
|b a G-protein interaction study
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|c 2006
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|b nc
|2 rdacarrier
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|a Date Completed 17.08.2007
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|a Date Revised 18.11.2010
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|a published: Print
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|a Citation Status MEDLINE
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|a The affinity of alpha(2A)-adrenergic receptor (alpha(2A)-AR) derived peptide adsorbates for the functional bovine brain G-protein is studied in the search for the minimum sequence recognition. Three short peptides (GPR-i2c, GPR-i3n, and GPR-i3c) are designed to mimic the second and third intracellular loops of the receptor. X-ray photoelectron spectroscopy is used to study the chemical composition of the peptides and the binding strength to the surfaces. Chemisorption of the peptides to the gold substrates is observed. Infrared spectroscopy is used to study the characteristic absorption bands of the peptides. The presence of peptides on the surfaces is verified by prominent amide I and amide II bands. The interaction between the peptides and the G-protein is studied with surface plasmon resonance. It is shown that GPR-i3n has the highest affinity for the G-protein. Equilibrium analysis of the binding shows that the G-protein keeps its native conformation when interacting with GPR-i3c, but during the interaction with GPR-i2c and GPR-i3n the conformation of G-protein is changed, leading to the formation of aggregates and/or multilayers
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Peptides
|2 NLM
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|a Receptors, Adrenergic, alpha-2
|2 NLM
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|a Gold
|2 NLM
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|a 7440-57-5
|2 NLM
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|a GTP-Binding Proteins
|2 NLM
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| 650 |
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|a EC 3.6.1.-
|2 NLM
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1 |
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|a Petoral, Rodrigo M
|c Jr
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lindell, Carina
|e verfasserin
|4 aut
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| 700 |
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|a Broo, Klas
|e verfasserin
|4 aut
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|a Uvdal, Kajsa
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 22(2006), 17 vom: 15. Aug., Seite 7260-4
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:22
|g year:2006
|g number:17
|g day:15
|g month:08
|g pages:7260-4
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