Effect of Rho kinase on hypoxia stimulated proliferation of pulmonary arterial smooth muscle cells
OBJECTIVE: To study whether Rho kinase is involved in the proliferation of pulmonary arterial smooth muscle cells (PASMCs) induced by hypoxia
| Veröffentlicht in: | Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue. - 1998. - 18(2006), 8 vom: 04. Aug., Seite 452-5 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , |
| Format: | Aufsatz |
| Sprache: | Chinese |
| Veröffentlicht: |
2006
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| Zugriff auf das übergeordnete Werk: | Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue |
| Schlagworte: | English Abstract Journal Article Research Support, Non-U.S. Gov't rho-Associated Kinases EC 2.7.11.1 |
| Zusammenfassung: | OBJECTIVE: To study whether Rho kinase is involved in the proliferation of pulmonary arterial smooth muscle cells (PASMCs) induced by hypoxia METHODS: Rat's PASMCs were isolated and cultured. Methyl thiazolyl tetrazolium (MTT) was used to determine the growth of cells. The cell cycle was analyzed by flow cytometry. Western blot was used to assess the expression of Rho kinase RESULTS: The results of MTT showed that the number of PASMCs was increased after exposure to low oxygen tension for 12 hours compared with PASMCs in normoxia. After exposure of PASMCs to hypoxia for 24 hours,the cellular proliferation peaked,and was higher than that of PASMCs exposed to normal oxygen tension, hypoxia and Y27632 (Rho kinase inhibitor) groups,then it declined when exposed for 48 hours. Analysis of cell cycle indicated that the ability of cell proliferation increased significantly in PASMCs exposed to hypoxia compared with normoxia. Rho kinase level was higher in PASMCs exposed to hypoxia compared with normoxia CONCLUSION: Hypoxia stimulates proliferation of PASMCs and promotes PASMCs into the phase of mitosis. The expression of Rho kinase is increased in PASMCs that are exposed to hypoxia, and it shows that Rho kinase is activated by hypoxia in PASMCs. Rho kinase maybe involve in the proliferation of PASMCs induced by hypoxia |
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| Beschreibung: | Date Completed 07.12.2010 Date Revised 04.08.2006 published: Print Citation Status MEDLINE |
| ISSN: | 1003-0603 |