Identification of a novel Cys2/His2-type zinc-finger protein as a component of a spermine-signaling pathway in tobacco

In a previous work, we identified a Cys(2)/His(2)-type zinc-finger transcription repressor, (ZFT1), that functions in a spermine-mediated signal transduction pathway in tobacco plants. Database search disclosed the presence of another Cys(2)/His(2)-type zinc-finger protein ZFP1 (accession number AAC...

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Veröffentlicht in:Journal of plant physiology. - 1979. - 164(2007), 6 vom: 01. Juni, Seite 785-93
1. Verfasser: Mitsuya, Yoshiko (VerfasserIn)
Weitere Verfasser: Takahashi, Yoshihiro, Uehara, Yukiko, Berberich, Thomas, Miyazaki, Atsushi, Takahashi, Hideki, Kusano, Tomonobu
Format: Aufsatz
Sprache:English
Veröffentlicht: 2007
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Guanidines Plant Proteins Polyamines Transcription Factors Green Fluorescent Proteins 147336-22-9 MDL 72527 1YVR349GN4 Spermine mehr... 2FZ7Y3VOQX Oxidoreductases EC 1.- pimagedine SCQ4EZQ113 Putrescine V10TVZ52E4
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245 1 0 |a Identification of a novel Cys2/His2-type zinc-finger protein as a component of a spermine-signaling pathway in tobacco 
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520 |a In a previous work, we identified a Cys(2)/His(2)-type zinc-finger transcription repressor, (ZFT1), that functions in a spermine-mediated signal transduction pathway in tobacco plants. Database search disclosed the presence of another Cys(2)/His(2)-type zinc-finger protein ZFP1 (accession number AAC06243) in tobacco plants. In this work, we characterized ZFP1 and investigated whether this protein is also involved in a Spm-signaling pathway. This factor showed the highest identity to petunia ZPT2-2 and higher similarity to petunia ZPT2-3, Arabidopsis STZ/ZAT10, soybean SCOF-1, red pepper CAZFP1/CaPIF1 as well as to tobacco ZFT1. ZFP1 localized to the nucleus and had a specific DNA-binding activity, supportive to be a transcription factor. Furthermore, the protein had a mild repression activity on transcription in plant cells. The expression of ZFP1, encoding ZFP1, was upregulated during tobacco mosaic virus-induced hypersensitive response. ZFP1 expression was also induced by exogenously applied spermine and its induction was repressed by inhibitors of amine oxidase/polyamine oxidase. Collectively, our data indicate that ZFP1 is a new transcription factor which functions in a spermine-signaling pathway in tobacco 
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700 1 |a Takahashi, Yoshihiro  |e verfasserin  |4 aut 
700 1 |a Uehara, Yukiko  |e verfasserin  |4 aut 
700 1 |a Berberich, Thomas  |e verfasserin  |4 aut 
700 1 |a Miyazaki, Atsushi  |e verfasserin  |4 aut 
700 1 |a Takahashi, Hideki  |e verfasserin  |4 aut 
700 1 |a Kusano, Tomonobu  |e verfasserin  |4 aut 
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