|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM164440763 |
| 003 |
DE-627 |
| 005 |
20231223102519.0 |
| 007 |
tu |
| 008 |
231223s2006 xx ||||| 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed24n0548.xml
|
| 035 |
|
|
|a (DE-627)NLM164440763
|
| 035 |
|
|
|a (NLM)16873449
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Morris, Wayne L
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Overexpression of a bacterial 1-deoxy-D-xylulose 5-phosphate synthase gene in potato tubers perturbs the isoprenoid metabolic network
|b implications for the control of the tuber life cycle
|
| 264 |
|
1 |
|c 2006
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
|
| 338 |
|
|
|a Band
|b nc
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 08.12.2006
|
| 500 |
|
|
|a Date Revised 10.03.2022
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Potato tubers were engineered to express a bacterial gene encoding 1-deoxy-D-xylulose 5-phosphate synthase (DXS) in order to investigate the effects of perturbation of isoprenoid biosynthesis. Twenty-four independent transgenic lines out of 38 generated produced tubers with significantly elongated shape that also exhibited an early tuber sprouting phenotype. Expression analysis of nine transgenic lines (four exhibiting the phenotype and five showing a wild-type phenotype) demonstrated that the phenotype was strongly associated with dxs expression. At harvest, apical bud growth had already commenced in dxs-expressing tubers whereas in control lines no bud growth was evident until dormancy was released after 56-70 d of storage. The initial phase of bud growth in dxs tubers was followed by a lag period of approximately 56 d, before further elongation of the developing sprouts could be detected. Thus dxs expression results in the separation of distinct phases in the dormancy and sprouting processes. In order to account for the sprouting phenotype, the levels of plastid-derived isoprenoid growth regulators were measured in transgenic and control tubers. The major difference measured was an increase in the level of trans-zeatin riboside in tubers at harvest expressing dxs. Additionally, compared with controls, in some dxs-expressing lines, tuber carotenoid content increased approximately 2-fold, with most of the increase accounted for by a 6-7-fold increase in phytoene
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
7 |
|a Bacterial Proteins
|2 NLM
|
| 650 |
|
7 |
|a Cytokinins
|2 NLM
|
| 650 |
|
7 |
|a Gibberellins
|2 NLM
|
| 650 |
|
7 |
|a Plant Growth Regulators
|2 NLM
|
| 650 |
|
7 |
|a Plant Proteins
|2 NLM
|
| 650 |
|
7 |
|a Terpenes
|2 NLM
|
| 650 |
|
7 |
|a Carotenoids
|2 NLM
|
| 650 |
|
7 |
|a 36-88-4
|2 NLM
|
| 650 |
|
7 |
|a Abscisic Acid
|2 NLM
|
| 650 |
|
7 |
|a 72S9A8J5GW
|2 NLM
|
| 650 |
|
7 |
|a Oxidoreductases
|2 NLM
|
| 650 |
|
7 |
|a EC 1.-
|2 NLM
|
| 650 |
|
7 |
|a phytoene dehydrogenase
|2 NLM
|
| 650 |
|
7 |
|a EC 1.14.99.-
|2 NLM
|
| 650 |
|
7 |
|a Transferases
|2 NLM
|
| 650 |
|
7 |
|a EC 2.-
|2 NLM
|
| 650 |
|
7 |
|a deoxyxylulose-5-phosphate synthase
|2 NLM
|
| 650 |
|
7 |
|a EC 2.2.1.-
|2 NLM
|
| 650 |
|
7 |
|a Alkyl and Aryl Transferases
|2 NLM
|
| 650 |
|
7 |
|a EC 2.5.-
|2 NLM
|
| 650 |
|
7 |
|a Geranylgeranyl-Diphosphate Geranylgeranyltransferase
|2 NLM
|
| 650 |
|
7 |
|a EC 2.5.1.32
|2 NLM
|
| 700 |
1 |
|
|a Ducreux, Laurence J M
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Hedden, Peter
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Millam, Steve
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Taylor, Mark A
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 57(2006), 12 vom: 15., Seite 3007-18
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
| 773 |
1 |
8 |
|g volume:57
|g year:2006
|g number:12
|g day:15
|g pages:3007-18
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 57
|j 2006
|e 12
|b 15
|h 3007-18
|