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DE-627 |
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20250207111425.0 |
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231223s2006 xx ||||| 00| ||chi c |
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|a pubmed25n0547.xml
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|a (DE-627)NLM164041613
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|a (NLM)16831244
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a chi
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| 100 |
1 |
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|a Li, Xu-yan
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Construction of a recombinant adenoviral vector carrying integrin-alpha v and the influence of integrin-alpha on the adhesion characteristics of SMMC-7721 cells
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| 264 |
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1 |
|c 2006
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 07.12.2010
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|a Date Revised 11.07.2006
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a OBJECTIVE: To establish human integrin-alpha v adenovirus expressed in human liver cancer SMMC-7721 cells and analyze the characteristics of integrin-alpha adhesion to liver cancer cells
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|a METHODS: Human integrin-alpha v cDNA was cloned into the plasmid pAdTrack-CMV. Then, pAdTrack-integrin-alpha v was cotransformed with adenoviral backbone vector into E. coli strain BJ5183. The recombinant adenoviral plasmid was identified by restriction analysis with Pac I and transfected into HEK-293A cells to package and amplify recombinant adenovirus particles. Western blot showed that integrin-alpha v gene was exactly transcript and expressed in SMMC-7721 cells. Twenty four hours after transfection, the effect of integrin-alpha on adhesion of SMMC-7721 cells was detected by adhesion experiment
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| 520 |
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|a RESULTS: Recombinant adenovirus vector of integrin-alpha v gene was successfully constructed and high titers of recombinant adenovirus was obtained. Adhesion cell count was significantly higher in integrin-alpha v transfected cells than in untransfected and Ad-null-transfected cells (P<0.01)
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| 520 |
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|a CONCLUSION: Over expression of integrin-alpha v promotes adhesion of SMMC-7721 cells, is an important molecular mechanism of tumor metastasis
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|a English Abstract
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a DNA, Complementary
|2 NLM
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| 650 |
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7 |
|a Integrin alphaV
|2 NLM
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| 700 |
1 |
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|a Liu, Jing-hua
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Zhi-jie
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mo, Yong-yan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Ya-wei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Zhi-feng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Deng, Peng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jiang, Yong
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 18(2006), 7 vom: 10. Juli, Seite 413-6
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
1 |
8 |
|g volume:18
|g year:2006
|g number:7
|g day:10
|g month:07
|g pages:413-6
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| 912 |
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 18
|j 2006
|e 7
|b 10
|c 07
|h 413-6
|