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NLM164039562 |
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DE-627 |
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20231223101636.0 |
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231223s2006 xx ||||| 00| ||eng c |
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|a pubmed24n0547.xml
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|a (DE-627)NLM164039562
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|a (NLM)16831017
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Schofield, Claire L
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Silver and gold glyconanoparticles for colorimetric bioassays
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| 264 |
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1 |
|c 2006
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 16.08.2007
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|a Date Revised 29.01.2022
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|a published: Print
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|a Citation Status MEDLINE
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|a The color changes associated with the aggregation of metal nanoparticles has led to the development of colorimetric-based assays for a variety of target species. We have examined both silver- and gold-based nanoparticles in order to establish whether either metal exhibits optimal characteristics for bioassay development. These silver and gold nanoparticles have been stabilized with a self-assembled monolayer of a mannose derivative (2-mercaptoethyl alpha-d-mannopyranoside) with the aim of inducing aggregation by exploiting the well-known interaction between mannose and the lectin Concanavalin A (Con A). Both metal glyconanoparticles were determined to be ca. 16 nm in diameter (using TEM measurements). Aggregation was observed on addition of Con A to both silver and gold nanoparticles resulting in a shift in the surface plasmon absorption band and a consequent color change of the solution, which was monitored using UV-visible spectrophotometry. Mannose-stabilized silver nanoparticles at a concentration of 3 nM provide an assay for Con A with the largest linear range (between 0.08 and 0.26 microM). Additionally, the kinetic rate of aggregation of the silver-nanoparticle-based bioassay was significantly greater than that of the gold-nanoparticle system. However, in terms of sensitivity, the mannose-stabilized gold-nanoparticle-based assay was optimum with a limit of detection of 0.04 microM Con A, as compared with a value of 0.1 microM obtained for the mannose-stabilized silver nanoparticles. Additionally, a lactose derivative (11-mercapto-3,6,9-trioxaundecyl beta-D-lactoside) was used to stabilize gold nanoparticles to induce aggregation upon addition of the galactose specific lectin Ricinus communis agglutinin (RCA(120)). To examine the specificity of the bioassay, lactose-stabilized gold nanoparticles were mixed with a solution of mannose-stabilized silver nanoparticles to give an aggregation assay capable of detecting two different lectins. When either Con A or RCA(120) was added to the mixed glyconanoparticles, selective recognition of the respective natural ligand was shown by aggregation of a single metal nanoparticle. Centrifugation and removal of the aggregated species enabled further bioassay measurements using the second glyconanoparticle system
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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7 |
|a Agglutinins
|2 NLM
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7 |
|a Glycoconjugates
|2 NLM
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| 650 |
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7 |
|a Ligands
|2 NLM
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7 |
|a Concanavalin A
|2 NLM
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| 650 |
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7 |
|a 11028-71-0
|2 NLM
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| 650 |
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7 |
|a Silver
|2 NLM
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| 650 |
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7 |
|a 3M4G523W1G
|2 NLM
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| 650 |
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7 |
|a Gold
|2 NLM
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| 650 |
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7 |
|a 7440-57-5
|2 NLM
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| 650 |
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7 |
|a Mannose
|2 NLM
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| 650 |
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|a PHA4727WTP
|2 NLM
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| 700 |
1 |
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|a Haines, Alan H
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Field, Robert A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Russell, David A
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 22(2006), 15 vom: 18. Juli, Seite 6707-11
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
8 |
|g volume:22
|g year:2006
|g number:15
|g day:18
|g month:07
|g pages:6707-11
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_22
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|a GBV_ILN_350
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|a GBV_ILN_721
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| 951 |
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|a AR
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| 952 |
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|d 22
|j 2006
|e 15
|b 18
|c 07
|h 6707-11
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