Drying of DNA droplets

The evaporation kinetics of droplets containing DNA was studied, as a function of DNA concentration. Drops containing very low DNA concentrations dried by maintaining a constant base, whereas those with high concentration dried with a constant contact angle. To understand this phenomenon, the distri...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1999. - 22(2006), 14 vom: 04. Juli, Seite 6308-12
1. Verfasser: Fang, Xiaohua (VerfasserIn)
Weitere Verfasser: Li, Bingquan, Petersen, Eric, Seo, Young-Soo, Samuilov, Vladimir A, Chen, Yong, Sokolov, Jonathan C, Shew, Chwen-Yang, Rafailovich, Miriam H
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, U.S. Gov't, Non-P.H.S. DNA, Viral
Beschreibung
Zusammenfassung:The evaporation kinetics of droplets containing DNA was studied, as a function of DNA concentration. Drops containing very low DNA concentrations dried by maintaining a constant base, whereas those with high concentration dried with a constant contact angle. To understand this phenomenon, the distribution of the DNA inside the droplet was measured using confocal microscopy. The results indicated that the DNA was condensed mostly on the surface of the droplets. In the case of high concentration droplets, it formed a shell, whereas isolated islands were found for droplets of low DNA concentrations. Rheologic results indicate the formation of a hydro gel in the low concentration drops, whereas phase separation between the self-assembled DNA structures and the water phase occurred at higher concentration
Beschreibung:Date Completed 03.08.2007
Date Revised 27.06.2006
published: Print
Citation Status MEDLINE
ISSN:1520-5827