Study of localization and translocation of human high mobility group protein B1 in eukaryotic cells
OBJECTIVE: To observe the localization and translocation of human high mobility group protein B1 (HMGB1) protein in eukaryotic cells
| Veröffentlicht in: | Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue. - 1998. - 18(2006), 6 vom: 20. Juni, Seite 338-41 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | Chinese |
| Veröffentlicht: |
2006
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| Zugriff auf das übergeordnete Werk: | Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue |
| Schlagworte: | English Abstract Journal Article Research Support, Non-U.S. Gov't HMGB1 Protein Recombinant Fusion Proteins enhanced green fluorescent protein Green Fluorescent Proteins 147336-22-9 |
| Zusammenfassung: | OBJECTIVE: To observe the localization and translocation of human high mobility group protein B1 (HMGB1) protein in eukaryotic cells METHODS: The vector that expressed the fusion protein of human HMGB1 and enhanced green fluorescent protein (EGFP) in mammalian cells were constructed. HMGB1 and EGFP were subcloned into hytohemagglutinin (HA) tagged vector pcDNA3-HA by two steps, the construct of which was then transfected into 293A cells and observed with fluorescence microscope RESULTS: The recombinant plasmid was verified by enzyme digestion, polymerase chain reaction (PCR) and sequence analysis, and the fusion protein was highly expressed in 293A cells. With fluorescence microscopy the green fluorescence was found to be distributed in the nuclei. Eighteen hours after the stimulation with tumor necrosis factor-alpha (TNF-alpha), it was found to have translocated into the cytoplasm in some of the cells CONCLUSION: The expression vector for HMGB1-EGFP fusion protein is successfully constructed and effectively expressed in mammalian cells, and it can serve as an important tool in the study of the signal pathway of HMGB1 in mammalian cells |
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| Beschreibung: | Date Completed 27.07.2007 Date Revised 20.06.2006 published: Print Citation Status MEDLINE |
| ISSN: | 1003-0603 |