Involvement of nitric oxide in light-mediated greening of barley seedlings

When seedlings are grown in the dark, proplastids of the developing leaf differentiate into etioplasts. Greening of etiolated plastids is stimulated by light, which is sensed by various types of photoreceptors. Nitric oxide (NO) has been shown to be a bioactive molecule that could take part in this...

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Veröffentlicht in:Journal of plant physiology. - 1979. - 163(2006), 8 vom: 30. Juli, Seite 818-26
1. Verfasser: Zhang, Lingang (VerfasserIn)
Weitere Verfasser: Wang, Yading, Zhao, Liqun, Shi, Suyun, Zhang, Lixin
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Cyclic N-Oxides Ferrocyanides Imidazoles Light-Harvesting Protein Complexes Membrane Proteins Photosystem I Protein Complex Plant Proteins Chlorophyll mehr... 1406-65-1 Nitroprusside 169D1260KM 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide 18390-00-6 Nitroarginine 2149-70-4 Nitric Oxide 31C4KY9ESH Nitric Oxide Synthase EC 1.14.13.39
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520 |a When seedlings are grown in the dark, proplastids of the developing leaf differentiate into etioplasts. Greening of etiolated plastids is stimulated by light, which is sensed by various types of photoreceptors. Nitric oxide (NO) has been shown to be a bioactive molecule that could take part in this light-mediated process in plants. In this paper, we show that emission of NO in barley seedlings increased concomitantly with increasing activities of nitric oxide synthase (NOS) during the greening. Treatment with sodium nitroprusside (SNP), a NO donor, increased the accumulation of chlorophyll contents, enhanced the accumulation of thylakoid membrane proteins, such as light harvesting complex of photosystem II (LHCII) and PSIA/B, and then improved the effective quantum yield of photosystem II (PSII) (Phi(PSII)) in the light. Instead, treatment with either NO scavenger 2-phenyl-4,4,5,5-tetramentylimidazoline-1-oxyl-3-xide (PTIO) or NOS inhibitor N(omega)-nitro-l-arginine (l-NNA) retarded the greening of etiolated-seedlings. Moreover, sodium ferrocyanide, an analog of SNP, nitrite and nitrate, two NO-decomposition products did not have any effect on the greening process. These results indicated that NO, as an endogenous signaling molecule, participates in light-mediated greening of barley seedlings, and exogenous NO accelerates this process 
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700 1 |a Wang, Yading  |e verfasserin  |4 aut 
700 1 |a Zhao, Liqun  |e verfasserin  |4 aut 
700 1 |a Shi, Suyun  |e verfasserin  |4 aut 
700 1 |a Zhang, Lixin  |e verfasserin  |4 aut 
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