Fluorescent aromatic platforms for cell patterning

This paper describes a simple experimental method of patterning fluorescent organic dyes, fluorescein, and rhodamine on gold substrates by microcontact printing techniques. The development of this step-by-step protocol has allowed us to prepare striped and squared micropatterns with poly(ethylene gl...

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Bibliographische Detailangaben
Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 22(2006), 13 vom: 20. Juni, Seite 5528-32
1. Verfasser: de la Fuente, Jesus M (VerfasserIn)
Weitere Verfasser: Andar, Abhay, Gadegaard, Nikolaj, Berry, Catherine C, Kingshott, Peter, Riehle, Mathis O
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Coated Materials, Biocompatible Fluorescent Dyes
Beschreibung
Zusammenfassung:This paper describes a simple experimental method of patterning fluorescent organic dyes, fluorescein, and rhodamine on gold substrates by microcontact printing techniques. The development of this step-by-step protocol has allowed us to prepare striped and squared micropatterns with poly(ethylene glycol) (PEG) linkers terminated by these fluorophores using a fast, easy, and inexpensive technique. Although the rest of the surface was covered with aliphatic molecules (methyl terminated), human fibroblasts demonstrated an unexpected response, aligning themselves according to the aromatic patterns, despite the presence of PEG, which is a cell resistant molecule, in the fluorescent regions
Beschreibung:Date Completed 20.07.2007
Date Revised 13.06.2006
published: Print
Citation Status MEDLINE
ISSN:1520-5827