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231223s2006 xx ||||| 00| ||eng c |
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|a (DE-627)NLM162947860
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|a (NLM)16714304
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Bartoli, Carlos G
|e verfasserin
|4 aut
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| 245 |
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|a Inter-relationships between light and respiration in the control of ascorbic acid synthesis and accumulation in Arabidopsis thaliana leaves
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| 264 |
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|c 2006
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 04.10.2006
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|a Date Revised 14.02.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The effects of growth irradiance and respiration on ascorbic acid (AA) synthesis and accumulation were studied in the leaves of wild-type and transformed Arabidopsis thaliana with modified amounts of the mitochondrial alternative oxidase (AOX) protein. Plants were grown under low (LL; 50 micromol photons m(-2) s(-1)), intermediate (IL; 100 micromol photons m(-2) s(-1)), or high (HL; 250 micromol photons m(-2) s(-1)) light. Increasing growth irradiance progressively elevated leaf AA content and hence the values of dark-induced disappearance of leaf AA, which were 11, 55, and 89 nmol AA lost g(-1) fresh weight h(-1), from LL-, IL-, and HL-grown leaves, respectively. When HL leaves were supplied with L-galactone-1,4-lactone (L-GalL; the precursor of AA), they accumulated twice as much AA and had double the maximal L-galactone-1,4-lactone dehydrogenase (L-GalLDH) activities of LL leaves. Growth under HL enhanced dehydroascorbate reductase and monodehydroascorbate reductase activities. Leaf respiration rates were highest in the HL leaves, which also had higher amounts of cytochrome c and cytochrome c oxidase (CCO) activities, as well as enhanced capacity of the AOX and CCO electron transport pathways. Leaves of the AOX-overexpressing lines accumulated more AA than wild-type or antisense leaves, particularly at HL. Intact mitochondria from AOX-overexpressing lines had higher AA synthesis capacities than those from the wild-type or antisense lines even though they had similar L-GalLDH activities. AOX antisense lines had more cytochrome c protein than wild-type or AOX-overexpressing lines. It is concluded that regardless of limitations on L-GalL synthesis by regulation of early steps in the AA synthesis pathway, the regulation of L-GalLDH activity via the interaction of light and respiratory controls is a crucial determinant of the overall ability of leaves to produce and accumulate AA
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Antioxidants
|2 NLM
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| 650 |
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|a Mitochondrial Proteins
|2 NLM
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Oxidoreductases
|2 NLM
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| 650 |
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|a EC 1.-
|2 NLM
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| 650 |
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|a alternative oxidase
|2 NLM
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| 650 |
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|a EC 1.-
|2 NLM
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| 650 |
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|a Electron Transport Complex IV
|2 NLM
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| 650 |
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|a EC 1.9.3.1
|2 NLM
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| 650 |
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|a Ascorbic Acid
|2 NLM
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| 650 |
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|a PQ6CK8PD0R
|2 NLM
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| 700 |
1 |
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|a Yu, Jianping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gómez, Facundo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fernández, Laura
|e verfasserin
|4 aut
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| 700 |
1 |
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|a McIntosh, Lee
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Foyer, Christine H
|e verfasserin
|4 aut
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| 773 |
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8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 57(2006), 8 vom: 21., Seite 1621-31
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:57
|g year:2006
|g number:8
|g day:21
|g pages:1621-31
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| 912 |
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|a GBV_USEFLAG_A
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|a AR
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|d 57
|j 2006
|e 8
|b 21
|h 1621-31
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