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231223s1997 xx ||||| 00| ||eng c |
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|a pubmed25n0543.xml
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|a (DE-627)NLM16280671X
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|a (NLM)16699242
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Schiltz, M
|e verfasserin
|4 aut
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|a Protein Crystallography at Ultra-Short Wavelengths
|b Feasibility Study of Anomalous-Dispersion Experiments at the Xenon K-edge
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|c 1997
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 02.10.2012
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|a Date Revised 15.05.2006
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a A protein crystallography experiment at the xenon K-edge (lambda = 0.358 A) has been successfully carried out at the materials science beamline (BL2/ID11) of the ESRF. The samples used in this methodological study were crystals of porcine pancreatic elastase, a 26 kDa protein of known structure. The diffraction data are of excellent quality. The combination of isomorphous replacement and anomalous dispersion of a single xenon heavy-atom derivative allowed accurate phase determination and the computation of a high-quality electron density map of the protein molecule. This is the first fully documented report on a complete protein crystallography experiment, from data collection up to phase determination and calculation of an electron density map, carried out with data obtained at ultra-short wavelengths. Experimental considerations as well as possible advantages and drawbacks of protein crystallography at very short and ultra-short wavelengths are discussed
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|a Journal Article
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|a Kvick, A
|e verfasserin
|4 aut
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|a Svensson, O S
|e verfasserin
|4 aut
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| 700 |
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|a Shepard, W
|e verfasserin
|4 aut
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| 700 |
1 |
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|a de La Fortelle, E
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Prangé, T
|e verfasserin
|4 aut
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| 700 |
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|a Kahn, R
|e verfasserin
|4 aut
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|a Bricogne, G
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fourme, R
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of synchrotron radiation
|d 1994
|g 4(1997), Pt 5 vom: 01. Sept., Seite 287-97
|w (DE-627)NLM09824129X
|x 0909-0495
|7 nnns
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| 773 |
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|g volume:4
|g year:1997
|g number:Pt 5
|g day:01
|g month:09
|g pages:287-97
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|d 4
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