Genetic and physiological characterization of tomato cv. Micro-Tom

Based on its compact habit, Micro-Tom, a dwarf cultivar of tomato (Solanum lycopersicum L.), has been proposed as a preferred variety to carry out molecular research in tomato. This cultivar, however, is poorly characterized. It is shown here that Micro-Tom has mutations in the SELF-PRUNING (SP) and...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 57(2006), 9 vom: 28., Seite 2037-47
1. Verfasser: Martí, Esmeralda (VerfasserIn)
Weitere Verfasser: Gisbert, Carmina, Bishop, Gerard J, Dixon, Mark S, García-Martínez, José L
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Gibberellins Plant Growth Regulators Plant Proteins SP protein, Lycopersicon esculentum Steroids
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520 |a Based on its compact habit, Micro-Tom, a dwarf cultivar of tomato (Solanum lycopersicum L.), has been proposed as a preferred variety to carry out molecular research in tomato. This cultivar, however, is poorly characterized. It is shown here that Micro-Tom has mutations in the SELF-PRUNING (SP) and DWARF (D) genes. In addition to this, it is also shown that Micro-Tom harbours at least two independently segregating resistance loci to the plant pathogen Cladosporium fulvum. The presence of the self-pruning mutation in Micro-Tom, that generates a determinate phenotype, was confirmed by crossing and sequence analysis. It was also found that Micro-Tom has a mutation in the DWARF gene (d) that leads to mis-splicing and production of at least two shorter mRNAs. The d mutation is predicted to generate truncated DWARF protein. The d sequence defect co-segregates with dark-green and rugose leaves, characteristics of brassinosteroid biosynthesis mutants. Micro-Tom also carries at least another mutation producing internode length reduction that affects plant height but not active gibberellin (GA) levels, which were similar in dwarf and tall Micro-TomxSeverianin segregants. GAs and brassinosteroids act synergistically in Micro-Tom, and the response to GA depends on brassinosteroids because the elongation of internodes was at least six times higher when GA(3) was applied simultaneously with brassinolide. A novel variety, Micro-0 that is fully susceptible to C. fulvum and almost as dwarf as Micro-Tom, has been generated from the cross of Cf0xMicro-Tom. This line represents a valuable resource for future analysis of Cf resistance genes through breeding or transformation 
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650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a Plant Growth Regulators  |2 NLM 
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700 1 |a Gisbert, Carmina  |e verfasserin  |4 aut 
700 1 |a Bishop, Gerard J  |e verfasserin  |4 aut 
700 1 |a Dixon, Mark S  |e verfasserin  |4 aut 
700 1 |a García-Martínez, José L  |e verfasserin  |4 aut 
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