Rapid development of polyclonal antisera against infectious salmon anaemia virus and its optimization and application as a diagnostic tool
Infectious salmon anaemia is an important disease of Atlantic salmon. One of the current methods of diagnosis is the indirect fluorescent antibody test (IFAT), using a monoclonal antibody specific to the haemagglutinin of the virus. The conformationally dependent nature of this antibody could be a d...
| Veröffentlicht in: | Journal of fish diseases. - 1998. - 29(2006), 5 vom: 15. Mai, Seite 293-300 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2006
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| Zugriff auf das übergeordnete Werk: | Journal of fish diseases |
| Schlagworte: | Comparative Study Journal Article Antibodies, Viral Immune Sera Formaldehyde 1HG84L3525 Alkaline Phosphatase EC 3.1.3.1 |
| Zusammenfassung: | Infectious salmon anaemia is an important disease of Atlantic salmon. One of the current methods of diagnosis is the indirect fluorescent antibody test (IFAT), using a monoclonal antibody specific to the haemagglutinin of the virus. The conformationally dependent nature of this antibody could be a drawback in its usefulness in other tests. This study describes the development and optimization of a polyclonal antiserum against infectious salmon anaemia virus, including a method of separating virus from cell culture components within culture supernatant. The antiserum was subsequently optimized for use in a variety of immunological diagnostic tests, including IFAT and an alkaline phosphatase-based immunoassay, and Western blot |
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| Beschreibung: | Date Completed 30.08.2007 Date Revised 30.09.2020 published: Print Citation Status MEDLINE |
| ISSN: | 0140-7775 |