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231223s2006 xx ||||| 00| ||eng c |
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|a (NLM)16677315
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Jutfelt, F
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Translocation of viable Aeromonas salmonicida across the intestine of rainbow trout, Oncorhynchus mykiss (Walbaum)
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| 264 |
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|c 2006
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 30.08.2007
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| 500 |
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|a Date Revised 30.09.2020
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|a published: Print
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|a Citation Status MEDLINE
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|a The pathogenic bacterium Aeromonas salmonicida is the causative agent of the destructive disease furunculosis in salmonids. Horizontal transmission in salmonids has been suggested to occur via the skin, gills and/or intestine. Previous reports are contradictory regarding the role of the intestine as a route of infection. The present study therefore investigates the possibility of bacterial translocation across intestinal epithelia using Ussing chamber technology, in vitro. Intestinal segments were exposed for 90 min to fluorescein isothiocyanate-labelled pathogenic A. salmonicida. Sampling from the serosal side of the Ussing chambers showed that bacteria were able to translocate across the intestinal epithelium in both the proximal and distal regions. Plating and subsequent colony counting showed that the bacteria were viable after translocation. During the 90 min exposure to A. salmonicida, the intestinal segments maintained high viability as measured by electrical parameters. The distal region responded to bacterial exposure by increasing the electrical resistance, indicating an increased mucus secretion. This study thus demonstrates translocation of live A. salmonicida through the intestinal epithelium of rainbow trout, suggesting that the intestine is a possible route of infection in salmonids
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| 650 |
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|a Comparative Study
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| 650 |
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|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a Carbon Radioisotopes
|2 NLM
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| 700 |
1 |
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|a Olsen, R E
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Glette, J
|e verfasserin
|4 aut
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| 700 |
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|a Ringø, E
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sundell, K
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 29(2006), 5 vom: 15. Mai, Seite 255-62
|w (DE-627)NLM098166034
|x 0140-7775
|7 nnns
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| 773 |
1 |
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|g volume:29
|g year:2006
|g number:5
|g day:15
|g month:05
|g pages:255-62
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|a AR
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| 952 |
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|d 29
|j 2006
|e 5
|b 15
|c 05
|h 255-62
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