Detection of high levels of recombination generated during PCR amplification of RNA templates

Recombination during the PCR amplification of DNA templates can be a serious problem for those seeking to genotype heterogeneous populations, yet a boon to those seeking to enhance variation during in vitro evolution. Here, the extent to which PCR generates chimeric full-length products was estimate...

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Veröffentlicht in:BioTechniques. - 1988. - 40(2006), 4 vom: 28. Apr., Seite 499-507
1. Verfasser: Yu, Wayne (VerfasserIn)
Weitere Verfasser: Rusterholtz, Karl J, Krummel, Amber T, Lehman, Niles
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, U.S. Gov't, Non-P.H.S. RNA 63231-63-0