|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM162043643 |
| 003 |
DE-627 |
| 005 |
20231223093420.0 |
| 007 |
tu |
| 008 |
231223s2006 xx ||||| 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed24n0540.xml
|
| 035 |
|
|
|a (DE-627)NLM162043643
|
| 035 |
|
|
|a (NLM)16618175
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Dai, Jinhua
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a High-capacity binding of proteins by poly(acrylic acid) brushes and their derivatives
|
| 264 |
|
1 |
|c 2006
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
|
| 338 |
|
|
|a Band
|b nc
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 03.07.2007
|
| 500 |
|
|
|a Date Revised 16.11.2017
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Polymeric coatings with high protein-binding capacities are important for increasing the output of affinity-based protein purification and decreasing the detection limits of antibody microarrays. This report describes the use of thick poly(acrylic acid) (PAA) brushes to immobilize as much as 80 monolayers of protein. The brushes were prepared using a recently developed procedure that allows polymerization of 100-nm-thick poly(tert-butyl acrylate) films from a surface in just 5 min along with hydrolysis of these films to PAA in 15 min. Covalent binding of bovine serum albumin (BSA) to PAA brushes that were activated using standard coupling agents, however, resulted in immobilization of less than two monolayers of BSA because of competitive hydrolysis of the esters in the activated film. In contrast, derivatization of PAA with nitrilotriacetate (NTA)-Cu2+ complexes yielded films capable of binding many monolayers of protein via metal-ion affinity interactions. For example, derivatization of 55-nm-thick PAA films with NTA-Cu2+ allowed immobilization of about 15 monolayers (5.8 microg/cm2 or 58 nm) of BSA. The binding capacity was even higher for myoglobin (7.7 microg/cm2) and anti-IgG (9.6 microg/cm2). Remarkably, electrostatic adsorption of lysozyme in 55-nm-thick, underivatized PAA resulted in as much as 80 monolayers (16.2 microg/cm2 or 162 nm) of adsorbed protein. Polymer synthesis, derivatization, and swelling, as well as BSA immobilization kinetics and thermodynamics were characterized using reflectance FT-IR spectroscopy, ellipsometry, and protein assays
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
|
| 650 |
|
7 |
|a Acrylic Resins
|2 NLM
|
| 650 |
|
7 |
|a Coated Materials, Biocompatible
|2 NLM
|
| 650 |
|
7 |
|a Proteins
|2 NLM
|
| 650 |
|
7 |
|a Serum Albumin, Bovine
|2 NLM
|
| 650 |
|
7 |
|a 27432CM55Q
|2 NLM
|
| 650 |
|
7 |
|a carbopol 940
|2 NLM
|
| 650 |
|
7 |
|a 4Q93RCW27E
|2 NLM
|
| 700 |
1 |
|
|a Bao, Zhiyi
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Sun, Lei
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Hong, Seong U
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Baker, Gregory L
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Bruening, Merlin L
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 22(2006), 9 vom: 25. Apr., Seite 4274-81
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
|
| 773 |
1 |
8 |
|g volume:22
|g year:2006
|g number:9
|g day:25
|g month:04
|g pages:4274-81
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_721
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 22
|j 2006
|e 9
|b 25
|c 04
|h 4274-81
|