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231223s2006 xx ||||| 00| ||eng c |
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|a (NLM)16548610
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Kim, Kwan
|e verfasserin
|4 aut
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|a Silver-particle-based surface-enhanced Raman scattering spectroscopy for biomolecular sensing and recognition
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|c 2006
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 06.06.2007
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|a Date Revised 24.11.2016
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|a published: Print
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|a Citation Status MEDLINE
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|a In this study, we demonstrate that 2-microm-sized Ag (microAg) powders can be used as a core material for constructing molecular sensing/recognition units operating via surface-enhanced Raman scattering (SERS). This is possible because microAg powders are very efficient substrates for both the infrared and Raman-spectroscopic characterization of molecular adsorbates prepared in a similar manner on silver surfaces; we can obtain an infrared spectrum of organic molecules adsorbed on microAg particles with a very high signal-to-noise ratio by diffuse reflectance infrared Fourier transform spectroscopy (DRIFT), and the Raman spectrum of organic monolayers on powdered silver is an SERS spectrum. The agglomeration of microAg particles in a highly concentrated buffer solution could be prevented by the layer-by-layer deposition of cationic and anionic polyelectrolytes such as poly(allylamine hydrochloride) (PAH) and poly(acrylic acid) (PAA). In fact, prior to depositing PAA and PAH, 4-aminobenzenethiol (4-ABT) was assembled on the surfaces of the microAg particles as SERS markers. Because of the presence of amine groups of 4-ABT, PAA could be readily deposited on the microAg particles. On the other hand, the outermost PAA layer could also be derivatized with biotin-derivatized poly(L-lysine). The nonspecific interaction of poly(L-lysine) with proteins could be suppressed by grafting poly(ethylene glycol) into the biotin-derivatized poly(L-lysine) molecules. On the basis of the nature of the SERS peaks of 4-ABT, it was confirmed that these biotinylated microAg powders were effective in selectively recognizing the streptavidin arrays. Because a number of different molecules can be used as SERS-marker molecules, such as probable 4-ABT, commercially available microAg powders must be a prospective material in molecular sensing/recognition, particularly via SERS
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Acrylic Resins
|2 NLM
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|a Coated Materials, Biocompatible
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|a Silver
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|a 3M4G523W1G
|2 NLM
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|a carbopol 940
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|2 NLM
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| 700 |
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|a Park, Hyoung Kun
|e verfasserin
|4 aut
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| 700 |
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|a Kim, Nam Hoon
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 22(2006), 7 vom: 28. März, Seite 3421-7
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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|g volume:22
|g year:2006
|g number:7
|g day:28
|g month:03
|g pages:3421-7
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