In vitro enzymatic oxidation of a fluorine-tagged sulfido substrate analogue : a 19F NMR investigation
2006 John Wiley & Sons, Ltd.
| Veröffentlicht in: | Magnetic resonance in chemistry : MRC. - 1985. - 44(2006), 6 vom: 24. Juni, Seite 629-32 |
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| Weitere Verfasser: | , , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2006
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| Zugriff auf das übergeordnete Werk: | Magnetic resonance in chemistry : MRC |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 9-fluoro-1-nonanol Fatty Acids Fatty Alcohols Fluorine Radioisotopes Sulfides Fluorine 284SYP0193 mehr... |
| Zusammenfassung: | 2006 John Wiley & Sons, Ltd. 1H-decoupled 19F NMR has been used to monitor the highly regioselective oxidation of a fluorine-tagged thia-fatty acid derivative by castor stearoyl-ACP delta9 desaturase. The major enzymatic product, after reductive work-up, was identified as 9-fluoro-1-nonanol. This compound could be easily distinguished from substrate and a 9-sulfoxy by-product on the basis of its 19F NMR chemical shift and spiking experiments using authentic standards. Structural assignment of the cleavage product was confirmed by GC-MS analysis of the enzymatic products |
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| Beschreibung: | Date Completed 05.09.2007 Date Revised 21.11.2013 published: Print Citation Status MEDLINE |
| ISSN: | 0749-1581 |