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|a eng
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| 100 |
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|a Carroll, Gregory T
|e verfasserin
|4 aut
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| 245 |
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|a Photochemical micropatterning of carbohydrates on a surface
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|c 2006
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|a Text
|b txt
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|a ohne Hilfsmittel zu benutzen
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|2 rdacarrier
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|a Date Completed 26.02.2007
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|a Date Revised 07.03.2006
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|a published: Print
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|a Citation Status MEDLINE
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|a In this report, we demonstrate a versatile method for the immobilization and patterning of unmodified carbohydrates onto glass substrates. The method employs a novel self-assembled monolayer to present photoactive phthalimide chromophores at the air-monolayer interface. Upon exposure to UV radiation, the phthalimide end-groups graft to surface-adsorbed carbohydrates, presumably by a hydrogen abstraction mechanism followed by radical recombination to form a covalent bond. Immobilized carbohydrate thin films are evidenced by fluorescence, ellipsometry and contact-angle measurements. Surface micropatterns of mono-, oligo-, and polysaccharides are generated by exposure through a contact photomask and are visualized by condensing water onto the surface. The efficiency of covalent coupling is dependent on the thermodynamic state of the surface. The amount of surface-grafted carbohydrate is enhanced when carbohydrate surface interactions are increased by the incorporation of amine-terminated molecules into the monolayer. Glass substrates modified with mixed monolayers of this nature are used to construct carbohydrate microarrays by spotting the carbohydrates with a robot and subsequently illuminating them with UV light to covalently link the carbohydrates. Surface-immobilized polysaccharides display well-defined antigenic determinants for antibody recognition. We demonstrate, therefore, that this novel technology combines the ability to create carbohydrate microarrays using the current state-of-the-art technology of robotic microspotting and the ability to control the shape of immobilized carbohydrate patterns with a spatial resolution defined by the UV wavelength and a shape defined by a photomask
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Carbohydrates
|2 NLM
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|a Wang, Denong
|e verfasserin
|4 aut
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|a Turro, Nicholas J
|e verfasserin
|4 aut
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|a Koberstein, Jeffrey T
|e verfasserin
|4 aut
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 22(2006), 6 vom: 14. März, Seite 2899-905
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|g volume:22
|g year:2006
|g number:6
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|g month:03
|g pages:2899-905
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