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231223s2006 xx ||||| 00| ||eng c |
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|a pubmed24n0537.xml
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|a (DE-627)NLM161106994
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|a (NLM)16519458
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Toscano, Alicia
|e verfasserin
|4 aut
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| 245 |
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|a Fibrinogen adsorption on three silica-based surfaces
|b conformation and kinetics
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|c 2006
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 26.02.2007
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|a Date Revised 19.10.2016
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|a published: Print
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|a Citation Status MEDLINE
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|a Using AFM (atomic force microscopy) to probe protein conformation and arrangement, and TIRF (total internal reflectance fluorescence) to monitor kinetics, fibrinogen adsorption on three different silica-based surfaces was studied: the native oxide on silicon, acid-etched microscope slides, and acid-etched polished glass. The three are chemically similar, but the microscope slide is rougher and induces AFM tip instabilities that appear as high spots on the bare surface. Fibrinogen's conformation and transport-limited adsorption kinetics are found to be quantitatively similar on all three surfaces. Further, the number of adsorbed proteins in progressive AFM micrographs quantitatively match the coverages measured by TIRF during early adsorption. Surfaces appear full, via AFM, when adsorbed amounts are about an order of magnitude below their true saturation levels (via TIRF) because, above about 0.26 mg/m(2), individual proteins cannot be discerned. The results demonstrate how the appearance of AFM micrographs can be misleading regarding surface saturation. On all three surfaces, fibrinogen is, at most, slightly aggregated, showing limited, if any, surface mobility. The complexities of the microscope slide's surface landscape minimally impact adsorption
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Silicon Dioxide
|2 NLM
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| 650 |
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|a 7631-86-9
|2 NLM
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| 650 |
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|a Fibrinogen
|2 NLM
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|a 9001-32-5
|2 NLM
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| 700 |
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|a Santore, Maria M
|e verfasserin
|4 aut
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 22(2006), 6 vom: 14. März, Seite 2588-97
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|x 1520-5827
|7 nnns
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| 773 |
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|g volume:22
|g year:2006
|g number:6
|g day:14
|g month:03
|g pages:2588-97
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