Differential toll-like receptor expression after ex vivo lipopolysaccharide exposure in patients with sepsis and following surgical stress

Differential toll-like receptor expression after ex vivo lipopolysaccharide exposure in patients with sepsis and following surgical stress

INTRODUCTION: Monocytes from septic patients have a reduced capacity to respond to lipopolysaccharide (LPS). We examined whether the same response occurred after surgical injury, and whether this reduced activity was associated with differential monocyte toll-like receptor (TLR) expression

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 119(2006), 2 vom: 01. Mai, Seite 180-7
1. Verfasser: Tsujimoto, Hironori (VerfasserIn)
Weitere Verfasser: Ono, Satoshi, Majima, Takashi, Efron, Philip A, Kinoshita, Manabu, Hiraide, Hoshio, Moldawer, Lyle L, Mochizuki, Hidetaka
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Comparative Study Journal Article Research Support, N.I.H., Extramural Antigens, CD Cytokines FCGR3B protein, human GPI-Linked Proteins HLA-DR Antigens Inflammation Mediators Lipopolysaccharide Receptors mehr... Lipopolysaccharides Receptors, IgG TLR2 protein, human TLR4 protein, human Toll-Like Receptor 2 Toll-Like Receptor 4 Toll-Like Receptors
Beschreibung
Zusammenfassung:INTRODUCTION: Monocytes from septic patients have a reduced capacity to respond to lipopolysaccharide (LPS). We examined whether the same response occurred after surgical injury, and whether this reduced activity was associated with differential monocyte toll-like receptor (TLR) expression
MATERIALS AND METHODS: Peripheral blood mononuclear cells (PBMCs) were isolated from septic patients, patients undergoing surgery, and healthy volunteers. Cells were stimulated ex vivo with LPS (1 microg/ml) and stained for CD14, CD16, TLR-2, TLR-4, and HLA-DR surface expression
RESULTS: TLR-2 and -4 expressions were significantly increased in monocytes from both septic and surgical patients. While ex vivo LPS-stimulation significantly increased TNFalpha and IL-1beta production in PBMCs from surgical patients, LPS-stimulation decreased IL-1beta production from septic patients as compared to surgical and control patients. Ex vivo LPS-stimulation induced TLR-4 upregulation in monocytes from both surgical and control patients, but not from septic patients. HLA-DR expression in CD14+CD16+ monocytes was reduced only in septic patients
CONCLUSIONS: PBMCs from septic patients, but not following surgical injury, have a reduced capacity to respond to a secondary inflammatory signal, but this defect is not associated with reduced TLR-4 or CD14 expression
Beschreibung:Date Completed 31.05.2006
Date Revised 16.11.2017
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035