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DE-627 |
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20250207042017.0 |
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tu |
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231223s2006 xx ||||| 00| ||eng c |
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|a pubmed25n0537.xml
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|a (DE-627)NLM16103411X
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|a (NLM)16511797
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Yamato, Osamu
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Use of amnion and placenta in neonatal screening for canine GM1-gangliosidosis and the risk of diagnostic misclassifications
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| 264 |
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|c 2006
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 05.05.2006
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|a Date Revised 09.11.2019
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a BACKGROUND: A closed breeding colony of Shiba dogs with GM1-gangliosidosis is maintained at Hokkaido University (Sapporo, Japan). Neonatal genotyping is essential to control the breeding colony genetically as an animal model for the human disease
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|a OBJECTIVES: The purpose of the present study was to determine the utility of amnion and placenta in the neonatal screening or diagnosis for canine GM1-gangliosidosis
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| 520 |
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|a METHODS: Twenty neonatal Shiba dogs of a pedigree with GM1- gangliosidosis were differentiated into 3 genotypes--normal, heterozygous, and affected dogs--by using a previously reported DNA mutation assay. Acid beta-galactosidase activity was measured in amnion and placenta and compared among the 3 genotypes
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|a RESULTS: The level of beta-galactosidase activity in the amnion of affected dogs was negligible and <2% of the mean activity in normal dogs; there was no significant difference among the 3 genotypes. In placenta, beta-galactosidase activity was significantly different among all the genotypes; however, there was wide overlap in enzyme activity between normal and heterozygous dogs. The level of activity in affected dogs was relatively high and >10% of the mean activity in normal dogs. The DNA mutation assay gave correct information about genotype with genomic DNA extracted from amnion but ambiguous information with DNA from placenta
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|a CONCLUSIONS: Amnion and placenta were not useful as enzyme sources in neonatal screening in canine GM1-gangliosidosis because of the risk of misdiagnosis. DNA from amnion is applicable as a template for genotyping, whereas placenta should not be used because canine placenta contains maternal cells
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a beta-Galactosidase
|2 NLM
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| 650 |
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7 |
|a EC 3.2.1.23
|2 NLM
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| 700 |
1 |
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|a Jo, Eun-Og
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Satoh, Hiroyuki
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yamauchi, Toyofumi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kobayashi, Asogi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yamasaki, Masahiro
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Maede, Yoshimitsu
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Veterinary clinical pathology
|d 1975
|g 35(2006), 1 vom: 01. März, Seite 91-4
|w (DE-627)NLM098159984
|x 0275-6382
|7 nnns
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| 773 |
1 |
8 |
|g volume:35
|g year:2006
|g number:1
|g day:01
|g month:03
|g pages:91-4
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| 912 |
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|a SYSFLAG_A
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| 912 |
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 35
|j 2006
|e 1
|b 01
|c 03
|h 91-4
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