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01000caa a22002652 4500 |
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NLM160673585 |
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DE-627 |
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20250207030041.0 |
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tu |
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231223s2006 xx ||||| 00| ||eng c |
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|a pubmed25n0536.xml
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|a (DE-627)NLM160673585
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|a (NLM)16473551
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Fuentes, P
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Inhibition of in vitro gamete adhesion and in vivo fertility in mice by immunization with a synthetic peptide
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| 264 |
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1 |
|c 2006
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 31.05.2006
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|a Date Revised 20.11.2014
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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| 520 |
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|a PROBLEM: To evaluate the contraceptive ability of a synthetic peptide in in vitro and in vivo fertility in the mouse
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| 520 |
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|a METHOD OF STUDY: A synthetic peptide segment: GELRERAPGQGTNG (SP) was used to immunize female B6CF1 (C57BL/6 x BALB/c) mice. A peptide with an amino acid sequence QQPLSIQQHERG (p2control) was used as control. Anti-SP and anti-p2control antisera were used to evaluate sperm function inhibition in vitro. Fertility of immunized mice was determined by microscopic evaluation of the number and state of preimplantation embryos (8-16 cell stage)
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|a RESULTS: In the mouse, anti-SP antisera recognized surface antigens in the acrosome region of mature and capacitated sperm. Anti-SP antisera inhibited in vitro sperm binding to zona pellucida. In vivo, immune response against SP in Freund's adjuvant resulted in a significant increase (P < 0.01) in the number of dead embryos and eggs (a mean of 66%, in contrast with < 25% in control mice). Fertility inhibition in vivo and in vitro was not observed when the p2control peptide was used in the immunizations
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|a CONCLUSIONS: These results would suggest that the SP sequence is involved in gamete adhesion, and an antifertility vaccine against the SP peptide segment could be feasible
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|a Journal Article
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Antibodies
|2 NLM
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| 650 |
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7 |
|a Immune Sera
|2 NLM
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| 650 |
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7 |
|a Peptides
|2 NLM
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| 700 |
1 |
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|a Palacio, J R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mayorga, M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Martínez, P
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 119(2006), 2 vom: 15. Mai, Seite 195-202
|w (DE-627)NLM098196855
|x 1521-6616
|7 nnns
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| 773 |
1 |
8 |
|g volume:119
|g year:2006
|g number:2
|g day:15
|g month:05
|g pages:195-202
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_11
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| 912 |
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|a GBV_ILN_24
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 119
|j 2006
|e 2
|b 15
|c 05
|h 195-202
|