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DE-627 |
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20231223090316.0 |
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tu |
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231223s2004 xx ||||| 00| ||eng c |
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|a pubmed24n0535.xml
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|a (DE-627)NLM160542022
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|a (NLM)16459604
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Huang, L C Lora
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Adsorption and immobilization of cytochrome c on nanodiamonds
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| 264 |
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1 |
|c 2004
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 20.03.2007
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| 500 |
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|a Date Revised 09.03.2022
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| 500 |
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a Methods have been developed to immobilize proteins onto the surfaces ofnanodiamonds with an average size of 5 +/- 1 nm. The immobilization started with carboxylation/oxidization of diamonds with strong acids, followed by coating the surfaces with poly-L-lysine (PL) for covalent attachment of proteins using heterobifunctional cross-linkers. The feasibility of this approach is proven with fluorescent labeling of the PL-coated diamonds by Alexa Fluor 488 and subsequent detection of the emission using a confocal fluorescence microscope. Immobilization of proteins onto the surfaces is also demonstrated with yeast cytochrome c, which possesses a free SH group for linkage and a characteristic Soret absorption band for observation
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| 650 |
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4 |
|a Journal Article
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a Enzymes, Immobilized
|2 NLM
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| 650 |
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7 |
|a Polylysine
|2 NLM
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| 650 |
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7 |
|a 25104-18-1
|2 NLM
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| 650 |
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7 |
|a Diamond
|2 NLM
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| 650 |
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7 |
|a 7782-40-3
|2 NLM
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| 650 |
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7 |
|a Cytochromes c
|2 NLM
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| 650 |
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7 |
|a 9007-43-6
|2 NLM
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| 700 |
1 |
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|a Chang, Huan-Cheng
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 20(2004), 14 vom: 06. Juli, Seite 5879-84
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
8 |
|g volume:20
|g year:2004
|g number:14
|g day:06
|g month:07
|g pages:5879-84
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_22
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| 912 |
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|a GBV_ILN_350
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| 912 |
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|a GBV_ILN_721
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| 951 |
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|a AR
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| 952 |
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|d 20
|j 2004
|e 14
|b 06
|c 07
|h 5879-84
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