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20231223090316.0 |
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231223s2004 xx ||||| 00| ||eng c |
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|a pubmed24n0535.xml
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|a (DE-627)NLM160542014
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|a (NLM)16459603
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Zhou, Cheng
|e verfasserin
|4 aut
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| 245 |
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|a Human immunoglobulin adsorption investigated by means of quartz crystal microbalance dissipation, atomic force microscopy, surface acoustic wave, and surface plasmon resonance techniques
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|c 2004
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 20.03.2007
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|a Date Revised 26.10.2019
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|a published: Print
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|a Citation Status MEDLINE
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|a Time-resolved adsorption behavior of a human immunoglobin G (hIgG) protein on a hydrophobized gold surface is investigated using multitechniques: quartz crystal microbalance/dissipation (QCM-D) technique; combined surface plasmon resonance (SPR) and Love mode surface acoustic wave (SAW) technique; combined QCM-D and atomic force microscopy (AFM) technique. The adsorbed hIgG forms interfacial structures varying in organization from a submonolayer to a multilayer. An "end-on" IgG orientation in the monolayer film, associated with the surface coverage results, does not corroborate with the effective protein thickness determined from SPR/SAW measurements. This inconsistence is interpreted by a deformation effect induced by conformation change. This conformation change is confirmed by QCM-D measurement. Combined SPR/SAW measurements suggest that the adsorbed protein barely contains water after extended contact with the hydrophobic surface. This limited interfacial hydration also contributed to a continuous conformation change in the adsorbed protein layer. The viscoelastic variation associated with interfacial conformation changes induces about 1.5 times overestimation of the mass uptake in the QCM-D measurements. The merit of combined multitechnique measurements is demonstrated
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Immunoglobulin G
|2 NLM
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|a Water
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|a 059QF0KO0R
|2 NLM
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|a Quartz
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|a 14808-60-7
|2 NLM
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|a Gold
|2 NLM
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|a 7440-57-5
|2 NLM
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| 700 |
1 |
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|a Friedt, Jean-Michel
|e verfasserin
|4 aut
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| 700 |
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|a Angelova, Angelina
|e verfasserin
|4 aut
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|a Choi, Kang-Hoon
|e verfasserin
|4 aut
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|a Laureyn, Wim
|e verfasserin
|4 aut
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| 700 |
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|a Frederix, Filip
|e verfasserin
|4 aut
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| 700 |
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|a Francis, Laurent A
|e verfasserin
|4 aut
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|a Campitelli, Andrew
|e verfasserin
|4 aut
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|a Engelborghs, Yves
|e verfasserin
|4 aut
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| 700 |
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|a Borghs, Gustaaf
|e verfasserin
|4 aut
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| 773 |
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8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 20(2004), 14 vom: 06. Juli, Seite 5870-8
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:20
|g year:2004
|g number:14
|g day:06
|g month:07
|g pages:5870-8
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_721
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|a AR
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|d 20
|j 2004
|e 14
|b 06
|c 07
|h 5870-8
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