Electrostatic orientation of enzymes on surfaces for ligand screening probed by force spectroscopy

Electrostatic orientation of enzymes on surfaces for ligand screening probed by force spectroscopy

In this letter, we show that electrostatic immobilization provides a simple but effective approach for the immobilization and orientation of carbonic anhydrase onto charged surfaces. The enzyme is oriented differently on oppositely charged surfaces, with the majority of active sites facing upward on...

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Bibliographische Detailangaben
Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 22(2006), 3 vom: 31. Jan., Seite 887-92
1. Verfasser: Wang, Xiuzhu (VerfasserIn)
Weitere Verfasser: Zhou, Dejian, Sinniah, Kumar, Clarke, Caroline, Birch, Louise, Li, Haitao, Rayment, Trevor, Abell, Chris
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Ligands Carbonic Anhydrase II EC 4.2.1.-