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231223s2006 xx ||||| 00| ||eng c |
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Trau, Dieter
|e verfasserin
|4 aut
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| 245 |
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|a Preservation of the biofunctionality of DNA and protein during microfabrication
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|c 2006
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 01.05.2007
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|a Date Revised 24.01.2006
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|a published: Print
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|a Citation Status MEDLINE
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|a Microfabrication processes, especially in silicon, are not compatible with biomolecules. Silicon and metal-based materials having crystalline structures are manipulated under harsh conditions with acids, bases, and organic solvents at high temperature. In comparison, organic biomolecules such as DNA and proteins have complex, three-dimensional structures and are sensitive to denaturation, oxidation, hydrolysis, and thermal destruction. Here, we report on the integration of DNA and the biotin-binding protein NeutrAvidin into microfabrication processes by using a novel approach based on a gold passivation mask. Our data show that this passivation method preserves approximately 84% of the biofunctionality of DNA and approximately 30% of that of NeutrAvidin under harsh process conditions. This novel technology enables the integration of DNA, proteins, and potentially other biological molecules into mass scalable microfabrication processes for biomedical devices, biochips, biosensors, and microelectromechanical systems with biomolecules (BioMEMS)
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Proteins
|2 NLM
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|a DNA
|2 NLM
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| 650 |
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|a 9007-49-2
|2 NLM
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| 700 |
1 |
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|a Jiang, Jie
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sucher, Nikolaus J
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1991
|g 22(2006), 3 vom: 31. Jan., Seite 877-81
|w (DE-627)NLM098181009
|x 0743-7463
|7 nnns
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| 773 |
1 |
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|g volume:22
|g year:2006
|g number:3
|g day:31
|g month:01
|g pages:877-81
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|d 22
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|h 877-81
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