Adsorption and fluctuations of giant liposomes studied by electrochemical impedance measurements

The present study describes a novel approach based on electrochemical impedance measurements to follow the adsorption of giant liposomes on protein-coated solid surfaces with a time resolution in the order of seconds. The technical key features are circular gold-film electrodes as small as a few hun...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1999. - 22(2006), 2 vom: 17. Jan., Seite 676-80
1. Verfasser: Sapper, Angelika (VerfasserIn)
Weitere Verfasser: Reiss, Björn, Janshoff, Andreas, Wegener, Joachim
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Comparative Study Journal Article Research Support, Non-U.S. Gov't Coated Materials, Biocompatible Liposomes Proteins Gold 7440-57-5
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245 1 0 |a Adsorption and fluctuations of giant liposomes studied by electrochemical impedance measurements 
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520 |a The present study describes a novel approach based on electrochemical impedance measurements to follow the adsorption of giant liposomes on protein-coated solid surfaces with a time resolution in the order of seconds. The technical key features are circular gold-film electrodes as small as a few hundred micrometers in diameter and measurements of the electrode capacitance using AC signals in the kilohertz regime. Using Monte Carlo simulations, we were able to support the experiments and extract the rate constant of liposome adsorption. Besides monitoring the adsorption of liposomes on protein-coated surfaces, we also applied this technique to study shape fluctuations of the adsorbed vesicles and compared the corresponding power spectra with those recorded for hard particles and living animal cells 
650 4 |a Comparative Study 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
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700 1 |a Reiss, Björn  |e verfasserin  |4 aut 
700 1 |a Janshoff, Andreas  |e verfasserin  |4 aut 
700 1 |a Wegener, Joachim  |e verfasserin  |4 aut 
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