Structure and mobility of lipid membranes on a thermoplastic substrate

Supported lipid membranes constitute one of the most important model systems for cell membranes. The properties of lipid membranes supported by the hydrophobic solid polymer cyclic olefin copolymer (COC) were investigated. Lipid layers consisting of varying amounts of 1,2-dioleoyl-3-trimethylammoniu...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 22(2006), 2 vom: 17. Jan., Seite 538-45
1. Verfasser: Hochrein, Marion B (VerfasserIn)
Weitere Verfasser: Reich, Christian, Krause, Bärbel, Rädler, Joachim O, Nickel, Bert
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Cations Cycloparaffins Fatty Acids, Monounsaturated Lipid Bilayers Membranes, Artificial Phosphatidylcholines Polymers Quaternary Ammonium Compounds mehr... Solvents 1,2-oleoylphosphatidylcholine EDS2L3ODLV 1,2-dioleoyloxy-3-(trimethylammonium)propane MR86K0XRQP
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100 1 |a Hochrein, Marion B  |e verfasserin  |4 aut 
245 1 0 |a Structure and mobility of lipid membranes on a thermoplastic substrate 
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520 |a Supported lipid membranes constitute one of the most important model systems for cell membranes. The properties of lipid membranes supported by the hydrophobic solid polymer cyclic olefin copolymer (COC) were investigated. Lipid layers consisting of varying amounts of 1,2-dioleoyl-3-trimethylammonium propane (DOTAP, cationic) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC, neutral) prepared by vesicle fusion and solvent exchange were compared. All lipid mixtures coated the COC surface homogeneously forming a fluid membrane as verified by fluorescence microscopy and fluorescence recovery after photobleaching (FRAP). The exact structure of the supported membranes was determined by synchrotron reflectivity experiments using a microfluidic chamber. The X-ray data are in agreement with a compressed (head-to-head distance = 29 angstroms) and less densely packed bilayer 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a Cycloparaffins  |2 NLM 
650 7 |a Fatty Acids, Monounsaturated  |2 NLM 
650 7 |a Lipid Bilayers  |2 NLM 
650 7 |a Membranes, Artificial  |2 NLM 
650 7 |a Phosphatidylcholines  |2 NLM 
650 7 |a Polymers  |2 NLM 
650 7 |a Quaternary Ammonium Compounds  |2 NLM 
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650 7 |a 1,2-oleoylphosphatidylcholine  |2 NLM 
650 7 |a EDS2L3ODLV  |2 NLM 
650 7 |a 1,2-dioleoyloxy-3-(trimethylammonium)propane  |2 NLM 
650 7 |a MR86K0XRQP  |2 NLM 
700 1 |a Reich, Christian  |e verfasserin  |4 aut 
700 1 |a Krause, Bärbel  |e verfasserin  |4 aut 
700 1 |a Rädler, Joachim O  |e verfasserin  |4 aut 
700 1 |a Nickel, Bert  |e verfasserin  |4 aut 
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