Expression and purification of Arisaema heterophyllum agglutinin in Escherichia coli
Recombinant Arisaema heterophyllum agglutinin (AHA) was expressed in Escherichia coli as N-terminal His-tagged fusions. After induction with isopropylthio-beta-D-galactoside, the recombinant AHA was purified by metal-affinity chromatography. The purified AHA protein was incorporated into artificial...
Veröffentlicht in: | Journal of plant physiology. - 1979. - 163(2006), 2 vom: 01. Feb., Seite 206-12 |
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1. Verfasser: | |
Weitere Verfasser: | , , , , |
Format: | Aufsatz |
Sprache: | English |
Veröffentlicht: |
2006
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Zugriff auf das übergeordnete Werk: | Journal of plant physiology |
Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Agglutinins Recombinant Fusion Proteins |