Cloning, characterization and expression of a Lateral suppressor-like gene from chrysanthemum (Dendranthema grandiflorum Kitamura)

Cloning, characterization and expression of a Lateral suppressor-like gene from chrysanthemum (Dendranthema grandiflorum Kitamura)

Conventionally, the lateral shoots of chrysanthemum are removed manually, which is time consuming and uneconomical. The development of branchless chrysanthemum will economize its commercial cultivation. To investigate the regulatory mechanism of branchlessness, we undertook cloning of Dendranthema g...

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Détails bibliographiques
Publié dans:Plant physiology and biochemistry : PPB. - 1991. - 43(2005), 12 vom: 15. Dez., Seite 1044-51
Auteur principal: Yang, Dae Hwa (Auteur)
Autres auteurs: Yun, Pil-Yong, Park, So Young, Plaha, Prikshit, Lee, Dong Sook, Lee, In Sok, Hwang, Yong Su, Kim, Young A, Lee, Jong Suk, Han, Bong Hee, Lee, Soo Young, Suh, Eun Jung, Lim, Yong Pyo
Format: Article
Langue:English
Publié: 2005
Accès à la collection:Plant physiology and biochemistry : PPB
Sujets:Journal Article Research Support, Non-U.S. Gov't Organophosphorus Compounds Plant Growth Regulators ethephon XU5R5VQ87S
Description
Résumé:Conventionally, the lateral shoots of chrysanthemum are removed manually, which is time consuming and uneconomical. The development of branchless chrysanthemum will economize its commercial cultivation. To investigate the regulatory mechanism of branchlessness, we undertook cloning of Dendranthema grandiflorum Kitamura Lateral suppressor-like (DgLsL) gene for development of lateral shoot in chrysanthemum. A full-length cDNA of DgLsL gene was isolated by screening cDNA library and performing Rapid Amplification of cDNA Ends (RACE) PCR. Phylogenetic analysis showed that the DgLsL gene is closely related to Lateral suppressor that encodes transcriptional regulator proteins belonging to GRAS family of known transcription factor. Southern blot analysis revealed that DgLsL gene in chrysanthemum genome has one copy. DgLsL expression was apparently up-regulated by ethephon treatment. The expression patterns revealed that DgLsL transcripts were detected in all organs, but showed their highest level in stems
Description:Date Completed 31.03.2006
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:0981-9428