Handling three regulatory elements in one transgene : combined use of cre-lox, FLP-FRT, and I-Scel recombination systems
Studies of regulatory systems in transgenic Drosophila are often compromised by possible genomic position effects on gene expression. As a result, it is desirable to be able to manipulate multiple regulatory elements in a single transgene construct. We developed an I-SceI endonuclease-based method t...
| Veröffentlicht in: | BioTechniques. - 1993. - 39(2005), 6 vom: 07. Dez., Seite 871-6 |
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| Format: | Aufsatz |
| Sprache: | English |
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2005
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Saccharomyces cerevisiae Proteins Viral Proteins Cre recombinase EC 2.7.7.- DNA Nucleotidyltransferases FLP recombinase Integrases SCEI protein, S cerevisiae mehr... |