Effect of sulfidogenic and methanogenic inhibitors on reductive dehalogenation of 2-chlorophenol

The potential for reductive dehalogenation of 2-CP in anaerobic batch cultures of fresh-water digested sludge under sulfidogenic and methanogenic conditions was investigated in the presence or absence of respective inhibitors: molybdate and BESA at various concentrations (0 to 10 mM). Triplicate cul...

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Veröffentlicht in:Environmental technology. - 1993. - 26(2005), 12 vom: 30. Dez., Seite 1383-91
1. Verfasser: Basu, S K (VerfasserIn)
Weitere Verfasser: Oleszkiewicz, J A, Sparling, R
Format: Aufsatz
Sprache:English
Veröffentlicht: 2005
Zugriff auf das übergeordnete Werk:Environmental technology
Schlagworte:Comparative Study Journal Article Research Support, Non-U.S. Gov't Chlorophenols Culture Media Halogens Propionates Sewage Sulfates molybdate mehr... 14259-85-9 Molybdenum 81AH48963U 2-chlorophenol K9KAV4K6BN Methane OP0UW79H66
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245 1 0 |a Effect of sulfidogenic and methanogenic inhibitors on reductive dehalogenation of 2-chlorophenol 
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520 |a The potential for reductive dehalogenation of 2-CP in anaerobic batch cultures of fresh-water digested sludge under sulfidogenic and methanogenic conditions was investigated in the presence or absence of respective inhibitors: molybdate and BESA at various concentrations (0 to 10 mM). Triplicate cultures (50% vol/vol) were set-up under an atmosphere of 20% CO2 and 80% N2 in 160 ml serum bottles using anaerobic digester sludge and a mineral medium containing 0.1% yeast extract. The dehalogenation of 2-CP, as well as methanogenesis, occurred at the same rate in the presence or absence of sulphate. Sulphate reduction did not inhibit 2-CP degrading populations. The presence of BESA--a known inhibitor of methane producers partially inhibited methanogenesis and slowed 2-CP dehalogenation at even 1 mM concentration with phenol and acetate accumulation in the cultures. The accumulation was proportional to the increase in concentration of BESA in the system. Molybdate on the other hand completely inhibited both sulphate reduction and 2-CP dehalogenation at a concentration of 10 mM. The dehalogenation of 2-CP continued in the presence of 1 mM molybdate even after the cessation of sulphate reduction indicating that sulphate-reducing bacteria were not directly involved in the dehalogenation of 2-CP in this study. Inhibition of 2-CP dehalogenation and sulphate reduction along with accumulation of propionate at 10 mM molybdate in the cultures strongly suggests that the dehalogenation of 2-CP was more directly linked to syntrophic activity of the mixed culture compared to sulphate reduction 
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650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a Halogens  |2 NLM 
650 7 |a Propionates  |2 NLM 
650 7 |a Sewage  |2 NLM 
650 7 |a Sulfates  |2 NLM 
650 7 |a molybdate  |2 NLM 
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650 7 |a Methane  |2 NLM 
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700 1 |a Oleszkiewicz, J A  |e verfasserin  |4 aut 
700 1 |a Sparling, R  |e verfasserin  |4 aut 
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