Role of asparagine and asparagine synthetase genes in sunflower (Helianthus annuus) germination and natural senescence

Sunflower (Helianthus annuus) contains three active asparagine synthetase (EC 6.3.5.4, AS) genes: HAS1, HAS1.1 and HAS2. Asparagine content and AS gene expression were determined during germination and leaf and cotyledon natural senescence to assess the role of asparagine as well as the extent of pa...

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Veröffentlicht in:Journal of plant physiology. - 1979. - 163(2006), 10 vom: 10. Okt., Seite 1061-70
1. Verfasser: Herrera-Rodríguez, María Begoña (VerfasserIn)
Weitere Verfasser: Maldonado, José María, Pérez-Vicente, Rafael
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Asparagine 7006-34-0 Aspartate-Ammonia Ligase EC 6.3.1.1
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520 |a Sunflower (Helianthus annuus) contains three active asparagine synthetase (EC 6.3.5.4, AS) genes: HAS1, HAS1.1 and HAS2. Asparagine content and AS gene expression were determined during germination and leaf and cotyledon natural senescence to assess the role of asparagine as well as the extent of participation of each AS gene in different nitrogen mobilizing processes. Asparagine accumulated in the dry seed and was the predominant amide throughout germination. During cotyledon senescence, the asparagine level was slightly higher than that of glutamine. The opposite was true for leaf senescence. According to transcript accumulation data, most of the asparagine newly synthesized for germination and cotyledon expansion was due to HAS2 activity, with little contribution of the other AS genes. However, all three genes work together to synthesize asparagine for leaf senescence. The absence of significant AS gene expression in cotyledon senescence differentiates leaf and cotyledon senescence, and suggests a cotyledon-specific regulation 
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