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231223s2005 xx ||||| 00| ||eng c |
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|a pubmed24n1324.xml
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|a (DE-627)NLM159451620
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|a (NLM)16343010
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Michel, Roger
|e verfasserin
|4 aut
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|a Influence of PEG architecture on protein adsorption and conformation
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|c 2005
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 19.07.2007
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|a Date Revised 12.03.2024
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|a published: Print
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|a Citation Status MEDLINE
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|a Poly(L-lysine)-g-poly(ethylene glycol) (PLL-g-PEG) copolymers with various grafting ratios were adsorbed to niobium pentoxide-coated silicon wafers and characterized before and after protein adsorption using X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS). Three proteins of different sizes, myoglobin (16 kD), albumin (67 kD), and fibrinogen (340 kD), were studied. XPS was used to quantify the amount of protein adsorbed to the bare and PEGylated surfaces. ToF-SIMS and principal component analysis (PCA) were used to study protein conformational changes on these surfaces. The smallest protein, myoglobin, generally adsorbed in higher numbers than the much larger fibrinogen. Protein adsorption was lowest on the surfaces with the highest PEG chain surface density and increased as the PEG layer density decreased. The highest adsorption was found on lysine-coated and bare niobium surfaces. ToF-SIMS and PCA data evaluation provided further information on the degree of protein denaturation, which, for a particular protein, were found to decrease with increasing PEG surface density and increase with decreasing protein size
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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| 650 |
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|a Proteins
|2 NLM
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|a Polyethylene Glycols
|2 NLM
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| 650 |
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|a 3WJQ0SDW1A
|2 NLM
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|a Pasche, Stephanie
|e verfasserin
|4 aut
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1 |
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|a Textor, Marcus
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Castner, David G
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 21(2005), 26 vom: 20. Dez., Seite 12327-32
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:21
|g year:2005
|g number:26
|g day:20
|g month:12
|g pages:12327-32
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|d 21
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|e 26
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|h 12327-32
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