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231223s2005 xx ||||| 00| ||eng c |
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|a pubmed24n1221.xml
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|a (NLM)16316753
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Lim, Hyoun-Sub
|e verfasserin
|4 aut
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| 245 |
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|a Soybean mosaic virus helper component-protease enhances somatic embryo production and stabilizes transgene expression in soybean
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| 264 |
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|c 2005
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 02.03.2006
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Soybean mosaic virus (SMV) helper component protease (HC-Pro), a suppressor of post-transcriptional gene silencing, was evaluated for its ability to enhance production of soybean hygromycin-resistant somatic embryos (HR-SEs), and stabilize transgene expression. Immature soybean cotyledonary explants were co-cultured with Agrobacterium tumefaciens strain KYRT1 harboring either pCAMBIA1302, carrying a hygromycin phosphotransferase gene (hpt) and a gene encoding green fluorescent protein; pCAMBIA1305.1, carrying hpt and beta-glucuronidase (uidA) genes; pG2-HC-Pro, a derivative of pCAMBIA1305.1 containing SMV G2 HC-Pro; or pG5-HC-Pro, a derivative of pCAMBIA1305.1 containing SMV G5 HC-Pro, but lacking uidA. Significantly (rho<0.02) higher numbers of HR-SEs were obtained from explants transformed with Agrobacterium harboring either pG2-HC-Pro or pG5-HC-Pro than with either of the vector controls (pCAMBIA1302 or pCAMBIA1305.1). Beta-glucuronidase (GUS) expression was significantly (rho<0.003) higher in 50-day-old transgenic plants expressing GUS along with SMV-HC-Pro and in SMV-infected GUS transgenic plants than in transgenic plants expressing GUS alone. Together, these data suggest that SMV-HC-Pro enhanced recovery of HR-SEs by suppressing silencing of the hygromycin phosphotransferase gene
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|a Journal Article
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a DNA Primers
|2 NLM
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|a DNA, Plant
|2 NLM
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|a RNA, Plant
|2 NLM
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|a Peptide Hydrolases
|2 NLM
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|a EC 3.4.-
|2 NLM
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|a Ko, Tae-Seok
|e verfasserin
|4 aut
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|a Lambert, Kris N
|e verfasserin
|4 aut
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| 700 |
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|a Kim, Hong-Gi
|e verfasserin
|4 aut
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| 700 |
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|a Korban, Schuyler S
|e verfasserin
|4 aut
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| 700 |
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|a Hartman, Glen L
|e verfasserin
|4 aut
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| 700 |
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|a Domier, Leslie L
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 43(2005), 10-11 vom: 01. Okt., Seite 1014-21
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
1 |
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|g volume:43
|g year:2005
|g number:10-11
|g day:01
|g month:10
|g pages:1014-21
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|d 43
|j 2005
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